6ACA

Crystal structure of Mycobacterium tuberculosis Mfd at 3.6 A resolution

PDB DOI: https://doi.org/10.2210/pdb6ACA/pdb

Classification: HYDROLASE
Organism(s): Mycobacterium tuberculosis H37Rv
Expression System: Escherichia coli
Mutation(s): No

Deposited: 2018-07-26 Released: 2019-08-28
Deposition Author(s): Putta, S., Fox, G.C., Walsh, M.A., Rao, D.N., Nagaraja, V., Natesh, R.
Funding Organization(s): Other government

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 3.60 Å
R-Value Free: 0.295
R-Value Work: 0.249
R-Value Observed: 0.252

wwPDB Validation 3D Report Full Report

This is version 1.1 of the entry. See complete history.

Literature

Structural basis for nucleotide-mediated remodelling mechanism of Mycobacterium Mfd

Putta, S., Prabha, S., Bhat, V., Fox, G.C., Walsh, M.A., Rao, D.N., Nagaraja, V., Natesh, R.

To be published.

Macromolecule Content

Total Structure Weight: 135.24 kDa
Atom Count: 8,275
Modelled Residue Count: 1,133
Deposited Residue Count: 1,254
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Mycobacterium tuberculosis Mfd	A	1,254	Mycobacterium tuberculosis H37Rv	Mutation(s): 0 Gene Names: mfd EC: 3.6.4
UniProt
Find proteins for P9WMQ5 (Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)) Explore P9WMQ5 Go to UniProtKB: P9WMQ5
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P9WMQ5
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 3.60 Å
R-Value Free: 0.295
R-Value Work: 0.249
R-Value Observed: 0.252
Space Group: P 4 3 2

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 224.259	α = 90
b = 224.259	β = 90
c = 224.259	γ = 90

Software Package:

Software Name	Purpose
PHENIX	refinement
iMOSFLM	data reduction
SCALA	data scaling
MOLREP	phasing

Structure Validation

View Full Validation Report

Entry History & Funding Information

Deposition Data

Released Date: 2019-08-28

Deposition Author(s):

Funding Organization	Location	Grant Number
Other government	India	BT/HRD/35/02/19/2009

Revision History (Full details and data files)

Version 1.0: 2019-08-28
Type: Initial release
Version 1.1: 2023-11-22
Changes: Data collection, Database references, Refinement description

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.