6AAY

the Cas13b binary complex


Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 2.79 Å
  • R-Value Free: 0.276 
  • R-Value Work: 0.221 
  • R-Value Observed: 0.224 

wwPDB Validation   3D Report Full Report


This is version 1.0 of the entry. See complete history


Literature

Structural insights into Cas13b-guided CRISPR RNA maturation and recognition.

Zhang, B.Ye, W.W.Ye, Y.M.Zhou, H.Saeed, A.F.U.H.Chen, J.Lin, J.Y.Perculija, V.Chen, Q.Chen, C.J.Chang, M.X.Choudhary, M.I.Ouyang, S.Y.

(2018) Cell Res 28: 1198-1201


Macromolecules

Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Bergeyella zoohelcum Cas13b (R1177A) mutant1,232Bergeyella zoohelcum ATCC 43767Mutation(s): 1 
Gene Names: HMPREF9699_02005
UniProt
Find proteins for K1LVU1 (Bergeyella zoohelcum ATCC 43767)
Explore K1LVU1 
Go to UniProtKB:  K1LVU1
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupK1LVU1
Sequence Annotations
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  • Reference Sequence
Find similar nucleic acids by:  (by identity cutoff)  |  3D Structure
Entity ID: 2
MoleculeChains LengthOrganismImage
RNA (52-MER)59Bergeyella zoohelcum
Sequence Annotations
Expand
  • Reference Sequence
Small Molecules
Modified Residues  1 Unique
IDChains TypeFormula2D DiagramParent
MSE
Query on MSE
A
L-PEPTIDE LINKINGC5 H11 N O2 SeMET
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 2.79 Å
  • R-Value Free: 0.276 
  • R-Value Work: 0.221 
  • R-Value Observed: 0.224 
  • Space Group: P 41 21 2
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 196.74α = 90
b = 196.74β = 90
c = 96.23γ = 90
Software Package:
Software NamePurpose
REFMACrefinement
xia2data reduction
xia2data scaling
PHENIXphasing

Structure Validation

View Full Validation Report



Entry History & Funding Information

Deposition Data


Funding OrganizationLocationGrant Number
National Science Foundation (China)China31770948

Revision History  (Full details and data files)

  • Version 1.0: 2019-03-13
    Type: Initial release