6XLI

CRYSTAL STRUCTURE OF ANTI-TAU ANTIBODY PT3 Fab+pT212/pT217-TAU PEPTIDE

Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 2.00 Å
  • R-Value Free: 0.213 
  • R-Value Work: 0.186 
  • R-Value Observed: 0.186 

wwPDB Validation   3D Report Full Report


This is version 1.2 of the entry. See complete history. 


Literature

Discovery and Functional Characterization of hPT3, a Humanized Anti-Phospho Tau Selective Monoclonal Antibody.

Van Kolen, K., Malia, T.J., Theunis, C., Nanjunda, R., Teplyakov, A., Ernst, R., Wu, S.J., Luo, J., Borgers, M., Vandermeeren, M., Bottelbergs, A., Wintmolders, C., Lacy, E., Maurin, H., Larsen, P., Willems, R., Van De Casteele, T., Triana-Baltzer, G., Slemmon, R., Galpern, W., Trojanowski, J.Q., Sun, H., Mercken, M.H.

(2020) J Alzheimers Dis 77: 1397-1416

  • DOI: https://doi.org/10.3233/JAD-200544
  • Primary Citation of Related Structures:  
    6XLI

  • PubMed Abstract: 

    As a consequence of the discovery of an extracellular component responsible for the progression of tau pathology, tau immunotherapy is being extensively explored in both preclinical and clinical studies as a disease modifying strategy for the treatment of Alzheimer's disease.

    • Organizational Affiliation: 

    Neuroscience Department, Janssen Research and Development, Beerse, Belgium.


Macromolecules
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(by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
PT3 Fab Heavy ChainA,
C,
E [auth H]		227Mus musculusMutation(s): 0 
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Sequence Annotations
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  • Reference Sequence
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Entity ID: 2
MoleculeChains Sequence LengthOrganismDetailsImage
PT3 Fab Light ChainB,
D,
F [auth L]		214Mus musculusMutation(s): 0 
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  • Reference Sequence

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Entity ID: 3
MoleculeChains Sequence LengthOrganismDetailsImage
Tau Phosphopeptide (Ac-SR(pT)PSLP(pT)PPTRE-OH)G [auth E],
H [auth F],
I [auth P]		14Homo sapiensMutation(s): 1 
UniProt & NIH Common Fund Data Resources
Find proteins for P10636 (Homo sapiens)
Explore P10636 
Go to UniProtKB:  P10636
PHAROS:  P10636
GTEx:  ENSG00000186868 
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP10636
Sequence Annotations
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  • Reference Sequence
Small Molecules
Modified Residues  1 Unique
IDChains TypeFormula2D DiagramParent
TPO
Query on TPO		G [auth E],
H [auth F],
I [auth P]		L-PEPTIDE LINKINGC4 H10 N O6 PTHR
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 2.00 Å
  • R-Value Free: 0.213 
  • R-Value Work: 0.186 
  • R-Value Observed: 0.186 
  • Space Group: C 1 2 1
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 126.24α = 90
b = 83.66β = 92.5
c = 166.87γ = 90
Software Package:
Software NamePurpose
PHENIXrefinement
XSCALEdata scaling
PDB_EXTRACTdata extraction
XDSdata reduction
PHASERphasing

Structure Validation

View Full Validation Report



View more in-depth experimental data
Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2020-09-23
    Type: Initial release
  • Version 1.1: 2020-10-28
    Changes: Database references
  • Version 1.2: 2023-10-18
    Changes: Data collection, Database references, Refinement description