Download MendeleyPuuD gamma-glutamyl-gamma-aminobutyrate hydrolase
Stogios, P.J.To be published.
6VTV
Crystal structure of PuuD gamma-glutamyl-gamma-aminobutyrate hydrolase from E. coli
- PDB DOI: https://doi.org/10.2210/pdb6VTV/pdb
- Classification: HYDROLASE
- Organism(s): Escherichia coli K-12
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2020-02-13 Released: 2020-02-26
- Funding Organization(s): National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.06 Å
- R-Value Free: 0.218
- R-Value Work: 0.173
- R-Value Observed: 0.176
wwPDB Validation 3D Report Full Report
Currently 6VTV does not have a validation slider image.
This is version 1.1 of the entry. See complete history.
Literature
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Gamma-glutamyl-gamma-aminobutyrate hydrolase PuuD | 258 | Escherichia coli K-12 | Mutation(s): 0 Gene Names: puuD, ycjL, b1298, JW1291 EC: 3.5.1.94 |  | |
UniProt | |||||
Find proteins for P76038 (Escherichia coli (strain K12)) Explore P76038 Go to UniProtKB: P76038 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P76038 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| MN (Subject of Investigation/LOI) Query on MN | C [auth A], D [auth B] | MANGANESE (II) ION Mn WAEMQWOKJMHJLA-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.06 Å
- R-Value Free: 0.218
- R-Value Work: 0.173
- R-Value Observed: 0.176
- Space Group: P 21 21 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 69.161 | α = 90 |
| b = 72.177 | β = 90 |
| c = 98.631 | γ = 90 |
| Software Name | Purpose |
|---|---|
| PHENIX | refinement |
| HKL-3000 | data reduction |
| HKL-3000 | data scaling |
| PHENIX | phasing |
Structure Validation
Currently 6VTV does not have a validation slider image.
Entry History & Funding Information
Deposition Data
- Released Date: 2020-02-26 Deposition Author(s): Stogios, P.J., EVDOKIMOVA, E., DI LEO, R., SAVCHENKO, A., JOACHIMIAK, A., SATCHELL, K.J.F., Center for Structural Genomics of Infectious Diseases (CSGID)
| Funding Organization | Location | Grant Number |
|---|---|---|
| National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) | United States | HHSN272201700060C |
Revision History (Full details and data files)
- Version 1.0: 2020-02-26
Type: Initial release - Version 1.1: 2023-10-11
Changes: Data collection, Database references, Derived calculations, Refinement description
