6V8R

Proteinase K Determined by MicroED Phased by ARCIMBOLDO_SHREDDER

  • Classification: HYDROLASE
  • Organism(s): Parengyodontium album
  • Expression System: Parengyodontium album
  • Mutation(s): No 

  • Deposited: 2019-12-11 Released: 2020-08-12 
  • Deposition Author(s): Richards, L.S., Martynowycz, M.W., Sawaya, M.R., Millan, C.
  • Funding Organization(s): National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS), National Science Foundation (NSF, United States), Department of Energy (DOE, United States), Sao Paulo Research Foundation (FAPESP), Spanish Ministry of Economy and Competitiveness

Experimental Data Snapshot

  • Method: ELECTRON CRYSTALLOGRAPHY
  • Resolution: 1.60 Å
  • R-Value Free: 0.232 
  • R-Value Work: 0.195 
  • R-Value Observed: 0.199 

wwPDB Validation   3D Report Full Report


This is version 1.0 of the entry. See complete history. 


Literature

Fragment-based determination of a proteinase K structure from MicroED data using ARCIMBOLDO_SHREDDER

Richards, L.R., Millan, C., Miao, J., Martynowycz, M.W., Sawaya, M.R., Gonen, T., Borges, R.J., Uson, I., Rodriguez, J.A.

(2020) Acta Crystallogr D Biol Crystallogr 76: 703-712


Macromolecules
Find similar proteins by: 
(by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Proteinase K279Parengyodontium albumMutation(s): 0 
Gene Names: PROK
EC: 3.4.21.64
UniProt
Find proteins for P06873 (Parengyodontium album)
Explore P06873 
Go to UniProtKB:  P06873
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP06873
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: ELECTRON CRYSTALLOGRAPHY
  • Resolution: 1.60 Å
  • R-Value Free: 0.232 
  • R-Value Work: 0.195 
  • R-Value Observed: 0.199 
  • Space Group: P 43 21 2
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 67.25α = 90
b = 67.25β = 90
c = 99.92γ = 90
Software Package:
Software NamePurpose
XDSdata reduction
XSCALEdata scaling
PHENIXrefinement
PDB_EXTRACTdata extraction
Arcimboldophasing

Structure Validation

View Full Validation Report



View more in-depth experimental data
Entry History & Funding Information

Deposition Data

Funding OrganizationLocationGrant Number
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)United States5T32GM008496
National Science Foundation (NSF, United States)United StatesDMR-1548924
Department of Energy (DOE, United States)United StatesDE-FC02-02ER63421
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)United StatesR35 GM128867
Sao Paulo Research Foundation (FAPESP)Brazil16/24191-8
Sao Paulo Research Foundation (FAPESP)Brazil17/13485-3
Spanish Ministry of Economy and CompetitivenessSpainBIO2015-64216-P
Spanish Ministry of Economy and CompetitivenessSpainPGC2018-101370-B-100
Spanish Ministry of Economy and CompetitivenessSpainMDM2014-0435-01

Revision History  (Full details and data files)

  • Version 1.0: 2020-08-12
    Type: Initial release