6UGU

Crystal structure of the Fab fragment of anti-TNFa antibody infliximab (Remicade) in a C-centered orthorhombic crystal form, Lot C

PDB DOI: https://doi.org/10.2210/pdb6UGU/pdb

Classification: IMMUNE SYSTEM
Organism(s): Homo sapiens
Expression System: Mus musculus
Mutation(s): No

Deposited: 2019-09-26 Released: 2019-11-13
Deposition Author(s): Lerch, T.F., Sharpe, P., Mayclin, S.J., Edwards, T.E., Polleck, S., Rouse, J.C., Conlan, H.D.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.20 Å
R-Value Free: 0.218
R-Value Work: 0.170
R-Value Observed: 0.172

wwPDB Validation 3D Report Full Report

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This is version 1.2 of the entry. See complete history.

Literature

Crystal Structures of PF-06438179/GP1111, an Infliximab Biosimilar.

Lerch, T.F., Sharpe, P., Mayclin, S.J., Edwards, T.E., Polleck, S., Rouse, J.C., Zou, Q., Conlon, H.D.

(2020) BioDrugs 34: 77-87

PubMed: 31650490 Search on PubMedSearch on PubMed Central
DOI: https://doi.org/10.1007/s40259-019-00390-1
Primary Citation of Related Structures:
6UGS, 6UGT, 6UGU, 6UGV

PubMed Abstract:
Higher-order structure (HOS) assessment is an important component of biosimilarity evaluations. While established spectroscopic methods are routinely used to characterize structure and evaluate similarity, the addition of X-ray crystallographic analysis to these biophysical methods enables orthogonal elucidation of HOS at higher resolution.

Organizational Affiliation

Analytical Research and Development, Biotherapeutics Pharmaceutical Sciences, Pfizer Inc., Chesterfield, MO, 63017, USA. thomas.lerch@pfizer.com.

Macromolecule Content

Total Structure Weight: 95.63 kDa
Atom Count: 7,400
Modelled Residue Count: 863
Deposited Residue Count: 880
Unique protein chains: 2

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
PF06438179 Fab Heavy Chain	A [auth H], C [auth A]	226	Homo sapiens	Mutation(s): 0
UniProt
Find proteins for A8K008 (Homo sapiens) Explore A8K008 Go to UniProtKB: A8K008
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	A8K008
Sequence Annotations Expand
Reference Sequence

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 2
Molecule	Chains	Sequence Length	Organism	Details	Image
PF06438179 Fab Light Chain	B [auth L], D [auth B]	214	Homo sapiens	Mutation(s): 0
UniProt
Find proteins for Q6P5S8 (Homo sapiens) Explore Q6P5S8 Go to UniProtKB: Q6P5S8
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q6P5S8
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.20 Å
R-Value Free: 0.218
R-Value Work: 0.170
R-Value Observed: 0.172
Space Group: C 2 2 2₁

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 87.26	α = 90
b = 138.15	β = 90
c = 195.31	γ = 90

Software Package:

Software Name	Purpose
XDS	data reduction
XSCALE	data scaling
PHENIX	refinement
PDB_EXTRACT	data extraction
PHASER	phasing

Structure Validation

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Currently 6UGU does not have a validation slider image.

Entry History

Deposition Data

Released Date: 2019-11-13

Deposition Author(s):

Revision History (Full details and data files)

Version 1.0: 2019-11-13
Type: Initial release
Version 1.1: 2020-02-12
Changes: Database references
Version 1.2: 2023-10-11
Changes: Data collection, Database references, Refinement description