6U79

Solution NMR structure of 5' UTR stem loop B from West Nile Virus


Experimental Data Snapshot

  • Method: SOLUTION NMR
  • Conformers Calculated: 150 
  • Conformers Submitted: 10 
  • Selection Criteria: structures with the lowest energy 

wwPDB Validation   3D Report Full Report


This is version 2.4 of the entry. See complete history


Literature

NMR structure of Dengue West Nile viruses stem-loop B: A key cis-acting element for flavivirus replication.

Sharma, S.Varani, G.

(2020) Biochem Biophys Res Commun 531: 522-527

  • DOI: https://doi.org/10.1016/j.bbrc.2020.07.115
  • Primary Citation of Related Structures:  
    6U79, 6W3M

  • PubMed Abstract: 

    Flaviviruses are major emerging human pathogenic viruses that pose a persistent and growing menace to global health. They are enveloped single-stranded RNA viruses with positive polarity transmitted by arthropod vectors like mosquitoes or ticks, responsible for a significant and growing number of human deaths and illnesses. The 5'- and 3'-untranslated regions (UTRs) are highly structured and contain conserved cis-acting RNA elements that participate in viral translation, replication and host adaptation. Despite their role in fiaviviruses replication, few high-resolution structural studies have investigated the RNA elements required for viral replication. Here we report the NMR structures of stem-loop B from WNV and DENV4 viruses. Because this element is required for cyclization of the genome and the activity of the replicative viral enzymes, viral replication rates are sensitive to even small changes in these RNAs. Therefore, this work provides structural insight into a new drug target to reduce flavivirus replication rates.


  • Organizational Affiliation

    Department of Chemistry, University of Washington, Seattle, WA, USA, 98195.


Macromolecules
Find similar nucleic acids by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains LengthOrganismImage
5' UTR region stem loop37West Nile virus
Sequence Annotations
Expand
  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: SOLUTION NMR
  • Conformers Calculated: 150 
  • Conformers Submitted: 10 
  • Selection Criteria: structures with the lowest energy 

Structure Validation

View Full Validation Report



Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2020-08-26
    Type: Initial release
  • Version 2.0: 2020-09-09
    Type: Coordinate replacement
    Reason: Atomic clashes
    Changes: Atomic model, Data collection, Database references, Derived calculations, Experimental preparation, Structure summary
  • Version 2.1: 2020-09-30
    Changes: Database references
  • Version 2.2: 2020-10-07
    Changes: Structure summary
  • Version 2.3: 2023-01-04
    Changes: Database references, Other, Structure summary
  • Version 2.4: 2023-06-14
    Changes: Other