6U5Z

Cryo-EM structure of E. coli LonA S679A

PDB DOI: https://doi.org/10.2210/pdb6U5Z/pdb
EM Map EMD-20659: EMDB EMDataResource

Classification: HYDROLASE
Organism(s): Escherichia coli
Expression System: Escherichia coli BL21(DE3)
Mutation(s): Yes

Deposited: 2019-08-28 Released: 2020-02-26
Deposition Author(s): Botos, I., Lountos, G.T., Weimin, W., Wlodawer, A.
Funding Organization(s): National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI), Russian Foundation for Basic Research

Experimental Data Snapshot

Method: ELECTRON MICROSCOPY
Resolution: 3.50 Å
Aggregation State: PARTICLE
Reconstruction Method: SINGLE PARTICLE

wwPDB Validation 3D Report Full Report

This is version 1.0 of the entry. See complete history.

Literature

Cryo-EM structure of substrate-free E. coli Lon protease provides insights into the dynamics of Lon machinery

Botos, I., Lountos, G.T., Weimin, W., Cherry, S., Ghirlando, R., Kudzhaev, A.M., Rotanova, T.V., de Val, N., Tropea, J., Gustchina, A., Wlodawer, A.

(2020) Curr Res Struct Biol 1: 13-20

DOI: https://doi.org/10.1016/j.crstbi.2019.10.001

Macromolecule Content

Total Structure Weight: 525.28 kDa
Atom Count: 24,606
Modelled Residue Count: 3,174
Deposited Residue Count: 4,704
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Lon protease	A, B, C, D, E A, B, C, D, E, F	784	Escherichia coli	Mutation(s): 1 Gene Names: lon, ACN002_0456, CV83915_01127, ECs0493, EL75_3311, EL80_3360 EC: 3.4.21.53
UniProt
Find proteins for P0A9M0 (Escherichia coli (strain K12)) Explore P0A9M0 Go to UniProtKB: P0A9M0
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P0A9M0
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: ELECTRON MICROSCOPY
Resolution: 3.50 Å
Aggregation State: PARTICLE
Reconstruction Method: SINGLE PARTICLE

EM Software:

Task	Software Package	Version
MODEL REFINEMENT	PHENIX	15
RECONSTRUCTION	RELION	3.0

Structure Validation

View Full Validation Report

Entry History & Funding Information

Deposition Data

Released Date: 2020-02-26

Deposition Author(s):

Funding Organization	Location	Grant Number
National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)	United States	HHSN261200800001E
Russian Foundation for Basic Research	Russian Federation	19-04-00646

Revision History (Full details and data files)

Version 1.0: 2020-02-26
Type: Initial release

Prepare Data

Validate Data

Deposit Data

Help and Resources

6U5Z

Cryo-EM structure of E. coli LonA S679A

Cryo-EM structure of substrate-free E. coli Lon protease provides insights into the dynamics of Lon machinery

Entity ID: 1

UniProt

Entity Groups

Sequence Annotations

Expand

Experimental Data

Structure Validation

Deposition Data

Revision History (Full details and data files)