6SWH

Crystal structure of the ternary complex between the type 1 pilus proteins FimC, FimI and FimA from E. coli


Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 2.80 Å
  • R-Value Free: 0.207 
  • R-Value Work: 0.171 
  • R-Value Observed: 0.171 

wwPDB Validation   3D Report Full Report


This is version 1.2 of the entry. See complete history


Literature

Comprehensive kinetic characterization of bacterial pilus rod assembly and assembly termination

Giese, C.Puorger, C.Ignatov, O.Weber, M.Scharer, M.A.Capitani, G.Glockshuber, R.

To be published.

Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Chaperone protein FimC
A, D
205Escherichia coli K-12Mutation(s): 0 
Gene Names: fimCb4316JW4279
UniProt
Find proteins for P31697 (Escherichia coli (strain K12))
Explore P31697 
Go to UniProtKB:  P31697
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP31697
Sequence Annotations
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  • Reference Sequence
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Entity ID: 2
MoleculeChains Sequence LengthOrganismDetailsImage
Fimbrin-like protein FimI
B, E
160Escherichia coli K-12Mutation(s): 0 
Gene Names: fimIb4315JW5779
UniProt
Find proteins for P39264 (Escherichia coli (strain K12))
Explore P39264 
Go to UniProtKB:  P39264
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP39264
Sequence Annotations
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  • Reference Sequence
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 3
MoleculeChains Sequence LengthOrganismDetailsImage
Type-1 fimbrial protein, A chain
C, F
153Escherichia coli K-12Mutation(s): 0 
Gene Names: fimApilAb4314JW4277
UniProt
Find proteins for P04128 (Escherichia coli (strain K12))
Explore P04128 
Go to UniProtKB:  P04128
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP04128
Sequence Annotations
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  • Reference Sequence
Small Molecules
Ligands 2 Unique
IDChains Name / Formula / InChI Key2D Diagram3D Interactions
PEG
Query on PEG

Download Ideal Coordinates CCD File 
G [auth A]DI(HYDROXYETHYL)ETHER
C4 H10 O3
MTHSVFCYNBDYFN-UHFFFAOYSA-N
EDO
Query on EDO

Download Ideal Coordinates CCD File 
H [auth B]1,2-ETHANEDIOL
C2 H6 O2
LYCAIKOWRPUZTN-UHFFFAOYSA-N
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 2.80 Å
  • R-Value Free: 0.207 
  • R-Value Work: 0.171 
  • R-Value Observed: 0.171 
  • Space Group: P 61
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 198.35α = 90
b = 198.35β = 90
c = 87.56γ = 120
Software Package:
Software NamePurpose
XSCALEdata scaling
PHENIXrefinement
PDB_EXTRACTdata extraction
XDSdata reduction
PHASERphasing

Structure Validation

View Full Validation Report



Entry History & Funding Information

Deposition Data


Funding OrganizationLocationGrant Number
Swiss National Science Foundation--

Revision History  (Full details and data files)

  • Version 1.0: 2020-10-07
    Type: Initial release
  • Version 1.1: 2020-10-28
    Changes: Derived calculations
  • Version 1.2: 2024-01-24
    Changes: Data collection, Database references, Refinement description