6NSF
Crystal structure of the A/Brisbane/10/2007 (H3N2) influenza virus hemagglutinin G186V/L194P mutant in complex with 3'-SLNLN
- PDB DOI: https://doi.org/10.2210/pdb6NSF/pdb
- Classification: VIRAL PROTEIN
- Organism(s): Influenza A virus (A/Brisbane/10/2007(H3N2))
- Expression System: Trichoplusia ni
- Mutation(s): Yes
- Deposited: 2019-01-24 Released: 2019-04-24
- Funding Organization(s): National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.10 Å
- R-Value Free: 0.214
- R-Value Work: 0.182
- R-Value Observed: 0.184
wwPDB Validation 3D Report Full Report
Currently 6NSF does not have a validation slider image.
This is version 2.1 of the entry. See complete history.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Hemagglutinin HA1 chain | 321 | Influenza A virus (A/Brisbane/10/2007(H3N2)) | Mutation(s): 1 Gene Names: HA |  | |
UniProt | |||||
Find proteins for A8W8K8 (Influenza A virus) Explore A8W8K8 Go to UniProtKB: A8W8K8 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | A8W8K8 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Hemagglutinin HA2 chain | 176 | Influenza A virus (A/Brisbane/10/2007(H3N2)) | Mutation(s): 0 Gene Names: HA |  | |
UniProt | |||||
Find proteins for A8W891 (Influenza A virus) Explore A8W891 Go to UniProtKB: A8W891 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | A8W891 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Oligosaccharides
Entity ID: 3 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Length | 2D Diagram | Glycosylation | 3D Interactions |
| alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | C | 5 |  | N-Glycosylation | |
Glycosylation Resources | |||||
GlyTouCan: G22768VO GlyCosmos: G22768VO GlyGen: G22768VO | |||||
Entity ID: 4 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Length | 2D Diagram | Glycosylation | 3D Interactions |
| 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[beta-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | D | 3 |  | N-Glycosylation | |
Glycosylation Resources | |||||
GlyTouCan: G28454KX GlyCosmos: G28454KX GlyGen: G28454KX | |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| SIA Query on SIA | I [auth A] | N-acetyl-alpha-neuraminic acid C11 H19 N O9 SQVRNKJHWKZAKO-YRMXFSIDSA-N |  | ||
| NAG Query on NAG | G [auth A], H [auth A], J [auth B] | 2-acetamido-2-deoxy-beta-D-glucopyranose C8 H15 N O6 OVRNDRQMDRJTHS-FMDGEEDCSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.10 Å
- R-Value Free: 0.214
- R-Value Work: 0.182
- R-Value Observed: 0.184
- Space Group: H 3 2
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 100.174 | α = 90 |
| b = 100.174 | β = 90 |
| c = 382.752 | γ = 120 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| HKL-2000 | data scaling |
| PHASER | phasing |
| HKL-2000 | data reduction |
Structure Validation
Currently 6NSF does not have a validation slider image.
Entry History & Funding Information
Deposition Data
- Released Date: 2019-04-24 Deposition Author(s): Wu, N.C., Wilson, I.A.
| Funding Organization | Location | Grant Number |
|---|---|---|
| National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) | United States | R56 AI127371 |
Revision History (Full details and data files)
- Version 1.0: 2019-04-24
Type: Initial release - Version 1.1: 2019-06-19
Changes: Data collection, Database references - Version 1.2: 2019-06-26
Changes: Data collection, Database references - Version 1.3: 2019-12-18
Changes: Author supporting evidence, Data collection - Version 2.0: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Atomic model, Data collection, Derived calculations, Structure summary - Version 2.1: 2023-10-11
Changes: Data collection, Database references, Derived calculations, Refinement description, Structure summary
