6N4F

The crystal structure of hemagglutinin from A/canine/IL/11613/2015 (H3N2) influenza virus.

PDB DOI: https://doi.org/10.2210/pdb6N4F/pdb

Classification: VIRAL PROTEIN
Organism(s): unidentified influenza virus
Expression System: Trichoplusia ni
Mutation(s): No

Deposited: 2018-11-19 Released: 2019-01-30
Deposition Author(s): Yang, H., Stevesn, J.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 3.01 Å
R-Value Free: 0.242
R-Value Work: 0.213
R-Value Observed: 0.215

wwPDB Validation 3D Report Full Report

This is version 1.0 of the entry. See complete history.

Literature

Assessment of Molecular, Antigenic, and Pathological Features of Canine Influenza A(H3N2) Viruses That Emerged in the United States.

Pulit-Penaloza, J.A., Simpson, N., Yang, H., Creager, H.M., Jones, J., Carney, P., Belser, J.A., Yang, G., Chang, J., Zeng, H., Thor, S., Jang, Y., Killian, M.L., Jenkins-Moore, M., Janas-Martindale, A., Dubovi, E., Wentworth, D.E., Stevens, J., Tumpey, T.M., Davis, C.T., Maines, T.R.

(2017) J Infect Dis 216: S499-S507

PubMed: 28934454 Search on PubMedSearch on PubMed Central
DOI: https://doi.org/10.1093/infdis/jiw620
Primary Citation of Related Structures:
6N4F

PubMed Abstract:
A single subtype of canine influenza virus (CIV), A(H3N8), was circulating in the United States until a new subtype, A(H3N2), was detected in Illinois in spring 2015. Since then, this CIV has caused thousands of infections in dogs in multiple states.

Organizational Affiliation

Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia.

Macromolecule Content

Total Structure Weight: 230.55 kDa
Atom Count: 15,352
Modelled Residue Count: 1,956
Deposited Residue Count: 2,064
Unique protein chains: 2

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Hemagglutinin HA1	A, C, E, G	334	unidentified influenza virus	Mutation(s): 0
UniProt
Find proteins for A0A218KIQ1 (Influenza A virus) Explore A0A218KIQ1 Go to UniProtKB: A0A218KIQ1
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	A0A218KIQ1
Sequence Annotations Expand
Reference Sequence

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 2
Molecule	Chains	Sequence Length	Organism	Details	Image
Hemagglutinin HA2	B, D, F, H	182	unidentified influenza virus	Mutation(s): 0
UniProt
Find proteins for A0A2U5FPI7 (Influenza A virus) Explore A0A2U5FPI7 Go to UniProtKB: A0A2U5FPI7
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	A0A2U5FPI7
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 3.01 Å
R-Value Free: 0.242
R-Value Work: 0.213
R-Value Observed: 0.215
Space Group: H 3

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 241.148	α = 90
b = 241.148	β = 90
c = 147.958	γ = 120

Software Package:

Software Name	Purpose
REFMAC	refinement
HKL-2000	data reduction
HKL-2000	data scaling
PHASER	phasing

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2019-01-30

Deposition Author(s):

Revision History (Full details and data files)

Version 1.0: 2019-01-30
Type: Initial release

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.