6LHO

The cryo-EM structure of coxsackievirus A16 empty particle in complex with Fab 18A7

  • Classification: VIRUS
  • Organism(s): Coxsackievirus A16
  • Mutation(s): No 

  • Deposited: 2019-12-09 Released: 2020-02-05 
  • Deposition Author(s): He, M.Z., Xu, L.F., Zheng, Q.B., Zhu, R., Yin, Z.C., Cheng, T., Li, S.W.
  • Funding Organization(s): National Natural Science Foundation of China, National Science Foundation (China), National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS), National Institutes of Health/National Institute of Dental and Craniofacial Research (NIH/NIDCR), National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)

Experimental Data Snapshot

  • Method: ELECTRON MICROSCOPY
  • Resolution: 3.13 Å
  • Aggregation State: PARTICLE 
  • Reconstruction Method: SINGLE PARTICLE 

wwPDB Validation   3D Report Full Report


This is version 1.3 of the entry. See complete history


Literature

Identification of Antibodies with Non-overlapping Neutralization Sites that Target Coxsackievirus A16.

He, M.Xu, L.Zheng, Q.Zhu, R.Yin, Z.Zha, Z.Lin, Y.Yang, L.Huang, Y.Ye, X.Li, S.Hou, W.Wu, Y.Han, J.Liu, D.Li, Z.Chen, Z.Yu, H.Que, Y.Wang, Y.Yan, X.Zhang, J.Gu, Y.Zhou, Z.H.Cheng, T.Li, S.Xia, N.

(2020) Cell Host Microbe 27: 249

  • DOI: https://doi.org/10.1016/j.chom.2020.01.003
  • Primary Citation of Related Structures:  
    6LHA, 6LHB, 6LHC, 6LHK, 6LHL, 6LHO, 6LHP, 6LHQ, 6LHT

  • PubMed Abstract: 

    Hand, foot, and mouth disease is a common childhood illness primarily caused by coxsackievirus A16 (CVA16), for which there are no current vaccines or treatments. We identify three CVA16-specific neutralizing monoclonal antibodies (nAbs) with therapeutic potential: 18A7, 14B10, and NA9D7. We present atomic structures of these nAbs bound to all three viral particle forms-the mature virion, A-particle, and empty particle-and show that each Fab can simultaneously occupy the mature virion. Additionally, 14B10 or NA9D7 provide 100% protection against lethal CVA16 infection in a neonatal mouse model. 18A7 binds to a non-conserved epitope present in all three particles, whereas 14B10 and NA9D7 recognize broad protective epitopes but only bind the mature virion. NA9D7 targets an immunodominant site, which may overlap the receptor-binding site. These findings indicate that CVA16 vaccines should be based on mature virions and that these antibodies could be used to discriminate optimal virion-based immunogens.


  • Organizational Affiliation

    State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Sciences, School of Public Health, Xiamen University, Xiamen 361102, China.


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
VP1 protein297Coxsackievirus A16Mutation(s): 0 
UniProt
Find proteins for A0A2S1BJ89 (Coxsackievirus A16)
Explore A0A2S1BJ89 
Go to UniProtKB:  A0A2S1BJ89
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupA0A2S1BJ89
Sequence Annotations
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  • Reference Sequence
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 2
MoleculeChains Sequence LengthOrganismDetailsImage
VP2 protein254Coxsackievirus A16Mutation(s): 0 
EC: 3.4.22.29 (PDB Primary Data), 3.6.1.15 (PDB Primary Data), 3.4.22.28 (PDB Primary Data), 2.7.7.48 (PDB Primary Data)
UniProt
Find proteins for Q9QF31 (Coxsackievirus A16 (strain Tainan/5079/98))
Explore Q9QF31 
Go to UniProtKB:  Q9QF31
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupQ9QF31
Sequence Annotations
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  • Reference Sequence
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 3
MoleculeChains Sequence LengthOrganismDetailsImage
VP3 protein242Coxsackievirus A16Mutation(s): 0 
EC: 3.4.22.29 (PDB Primary Data), 3.6.1.15 (PDB Primary Data), 3.4.22.28 (PDB Primary Data), 2.7.7.48 (PDB Primary Data)
UniProt
Find proteins for Q9QF31 (Coxsackievirus A16 (strain Tainan/5079/98))
Explore Q9QF31 
Go to UniProtKB:  Q9QF31
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupQ9QF31
Sequence Annotations
Expand
  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: ELECTRON MICROSCOPY
  • Resolution: 3.13 Å
  • Aggregation State: PARTICLE 
  • Reconstruction Method: SINGLE PARTICLE 

Structure Validation

View Full Validation Report



Entry History & Funding Information

Deposition Data


Funding OrganizationLocationGrant Number
National Natural Science Foundation of ChinaChina81991490
National Science Foundation (China)China2018ZX09711003-005-003
National Science Foundation (China)China2017ZX10304402-002-003
National Natural Science Foundation of ChinaChina31670933
National Natural Science Foundation of ChinaChina81801646
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)United StatesR37-GM33050
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)United StatesGM071940
National Institutes of Health/National Institute of Dental and Craniofacial Research (NIH/NIDCR)United StatesDE025567
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)United StatesAI094386

Revision History  (Full details and data files)

  • Version 1.0: 2020-02-05
    Type: Initial release
  • Version 1.1: 2020-02-19
    Changes: Database references
  • Version 1.2: 2020-02-26
    Changes: Database references
  • Version 1.3: 2022-03-23
    Changes: Author supporting evidence, Database references, Derived calculations