Download MendeleyCharacterisation of a monoclonal antibody conformationally-selective for native alpha-1-antitrypsin
Elliston, E.L.K., Miranda, E., Perez, J., Irving, J.A., Lomas, D.A.To be published.
6GXX
Fab fragment of an antibody selective for alpha-1-antitrypsin in the native conformation
- PDB DOI: https://doi.org/10.2210/pdb6GXX/pdb
- Classification: PROTEIN BINDING
- Organism(s): Mus musculus
- Mutation(s): No
- Deposited: 2018-06-27 Released: 2019-07-10
- Funding Organization(s): Medical Research Council (United Kingdom), Wellcome Trust, Other private, Other government
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.85 Å
- R-Value Free: 0.223
- R-Value Work: 0.186
- R-Value Observed: 0.188
wwPDB Validation 3D Report Full Report
This is version 1.1 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| FAB 1D9 heavy chain | A [auth H] | 226 | Mus musculus | Mutation(s): 0 |  |
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
Sequence AnnotationsExpand | |||||
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Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| FAB 1D9 light chain | B [auth L] | 217 | Mus musculus | Mutation(s): 0 |  |
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| MG Query on MG | C [auth L] | MAGNESIUM ION Mg JLVVSXFLKOJNIY-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.85 Å
- R-Value Free: 0.223
- R-Value Work: 0.186
- R-Value Observed: 0.188
- Space Group: P 2 21 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 54.103 | α = 90 |
| b = 85.257 | β = 90 |
| c = 89.427 | γ = 90 |
| Software Name | Purpose |
|---|---|
| PHENIX | refinement |
| XDS | data reduction |
| Aimless | data scaling |
| PHASER | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2019-07-10 Deposition Author(s): Elliston, E.L.K., Miranda, E., Perez, J., Lomas, D.A., Irving, J.A.
| Funding Organization | Location | Grant Number |
|---|---|---|
| Medical Research Council (United Kingdom) | United Kingdom | MR/N024842/1 |
| Wellcome Trust | United Kingdom | Wellcome Trust 4-year PhD Interdisciplinary Programme in Structural, Computational and Chemical Biology |
| Other private | United States | Alpha-1 Foundation project grant to James Irving |
| Other private | United States | Alpha-1 Foundation pilot grant to Elena Miranda |
| Other government | United Kingdom | UCLH/NIHR Biomedical Research Centre |
Revision History (Full details and data files)
- Version 1.0: 2019-07-10
Type: Initial release - Version 1.1: 2024-01-17
Changes: Data collection, Database references, Refinement description
