5ZY5

spCOMT apo structure

PDB DOI: https://doi.org/10.2210/pdb5ZY5/pdb

Classification: TRANSFERASE
Organism(s): Schizosaccharomyces pombe 972h-
Expression System: Escherichia coli K-12
Mutation(s): No

Deposited: 2018-05-22 Released: 2019-05-22
Deposition Author(s): Wang, Q., Xu, L.
Funding Organization(s): National Natural Science Foundation of China

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.29 Å
R-Value Free: 0.246
R-Value Work: 0.194
R-Value Observed: 0.196

wwPDB Validation 3D Report Full Report

This is version 1.1 of the entry. See complete history.

Literature

structural and functional investigations of spCOMT

Wang, Q., Li, X.

To be published.

Macromolecule Content

Total Structure Weight: 62.83 kDa
Atom Count: 3,920
Modelled Residue Count: 481
Deposited Residue Count: 548
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Probable catechol O-methyltransferase 1	A, B	274	Schizosaccharomyces pombe 972h-	Mutation(s): 0 Gene Names: SPBC119.03 EC: 2.1.1.6
UniProt
Find proteins for O42898 (Schizosaccharomyces pombe (strain 972 / ATCC 24843)) Explore O42898 Go to UniProtKB: O42898
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	O42898
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.29 Å
R-Value Free: 0.246
R-Value Work: 0.194
R-Value Observed: 0.196
Space Group: P 2₁ 2₁ 2₁

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 73.42	α = 90
b = 79.792	β = 90
c = 104.519	γ = 90

Software Package:

Software Name	Purpose
PHENIX	refinement
HKL-2000	data reduction
HKL-2000	data scaling
MOLREP	phasing

Structure Validation

View Full Validation Report

Entry History & Funding Information

Deposition Data

Released Date: 2019-05-22

Deposition Author(s):

Funding Organization	Location	Grant Number
National Natural Science Foundation of China	China	U1732114, 31770788 and 31770895

Revision History (Full details and data files)

Version 1.0: 2019-05-22
Type: Initial release
Version 1.1: 2023-11-22
Changes: Data collection, Database references, Refinement description

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.