5ZG8

Crystal Structure of TtNRS

PDB DOI: https://doi.org/10.2210/pdb5ZG8/pdb

Classification: LIGASE
Organism(s): Thermus thermophilus
Expression System: Escherichia coli BL21(DE3)
Mutation(s): No

Deposited: 2018-03-08 Released: 2019-03-13
Deposition Author(s): Mutharasappan, N., Jain, V., Sharma, A., Manickam, Y., Jeyaraman, J.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.40 Å
R-Value Free: 0.209
R-Value Work: 0.155
R-Value Observed: 0.158

wwPDB Validation 3D Report Full Report

This is version 1.1 of the entry. See complete history.

Literature

Crystal Structure of TtNRS

Mutharasappan, N., Jain, V., Sharma, A., Manickam, Y., Jeyaraman, J.

To be published.

Macromolecule Content

Total Structure Weight: 50.86 kDa
Atom Count: 3,627
Modelled Residue Count: 422
Deposited Residue Count: 438
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Asparagine--tRNA ligase	A	438	Thermus thermophilus	Mutation(s): 0 EC: 6.1.1.22
UniProt
Find proteins for P54263 (Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)) Explore P54263 Go to UniProtKB: P54263
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P54263
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.40 Å
R-Value Free: 0.209
R-Value Work: 0.155
R-Value Observed: 0.158
Space Group: P 3₁ 2 1

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 67.294	α = 90
b = 67.294	β = 90
c = 189.117	γ = 120

Software Package:

Software Name	Purpose
AMoRE	phasing
REFMAC	refinement
PDB_EXTRACT	data extraction
MOSFLM	data reduction
SCALA	data scaling

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2019-03-13

Deposition Author(s):

Mutharasappan, N.

Revision History (Full details and data files)

Version 1.0: 2019-03-13
Type: Initial release
Version 1.1: 2023-11-22
Changes: Data collection, Database references, Refinement description

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.