5XS9

Crystal structure of Mycobacterium smegmatis BioQ

PDB DOI: https://doi.org/10.2210/pdb5XS9/pdb

Classification: TRANSCRIPTION
Organism(s): Mycolicibacterium smegmatis MC2 155
Expression System: Escherichia coli
Mutation(s): No

Deposited: 2017-06-13 Released: 2018-06-27
Deposition Author(s): Zhang, Y., Ji, Q., Feng, Y.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 1.90 Å
R-Value Free: 0.229
R-Value Work: 0.179
R-Value Observed: 0.182

wwPDB Validation 3D Report Full Report

This is version 1.1 of the entry. See complete history.

Literature

Structural analysis of BioQ suggests a distinct regulatory mechanism for biotin, a nutritional virulence factor in Mycobacterium

Wei, W., Zhang, Y., Gao, R., Li, J., Xu, Y., Wang, S., Ji, Q., Feng, Y.

To be published.

Macromolecule Content

Total Structure Weight: 44.41 kDa
Atom Count: 2,916
Modelled Residue Count: 355
Deposited Residue Count: 410
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
TetR family transcriptional regulator	A, B	205	Mycolicibacterium smegmatis MC2 155	Mutation(s): 0 Gene Names: MSMEI_3111
UniProt
Find proteins for A0QX69 (Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)) Explore A0QX69 Go to UniProtKB: A0QX69
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	A0QX69
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 1.90 Å
R-Value Free: 0.229
R-Value Work: 0.179
R-Value Observed: 0.182
Space Group: C 2 2 2₁

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 61.041	α = 90
b = 190.179	β = 90
c = 83.413	γ = 90

Software Package:

Software Name	Purpose
REFMAC	refinement
HKL-3000	data reduction
HKL-3000	data scaling
PHASER	phasing

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2018-06-27

Deposition Author(s):

Revision History (Full details and data files)

Version 1.0: 2018-06-27
Type: Initial release
Version 1.1: 2024-03-27
Changes: Data collection, Database references

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.