5WDD

Crystal structure of chicken BOK


Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.80 Å
  • R-Value Free: 0.232 
  • R-Value Work: 0.195 
  • R-Value Observed: 0.197 

wwPDB Validation   3D Report Full Report


This is version 1.3 of the entry. See complete history


Literature

Embryogenesis and Adult Life in the Absence of Intrinsic Apoptosis Effectors BAX, BAK, and BOK.

Ke, F.F.S.Vanyai, H.K.Cowan, A.D.Delbridge, A.R.D.Whitehead, L.Grabow, S.Czabotar, P.E.Voss, A.K.Strasser, A.

(2018) Cell 173: 1217-1230.e17

  • DOI: https://doi.org/10.1016/j.cell.2018.04.036
  • Primary Citation of Related Structures:  
    5WDD

  • PubMed Abstract: 

    Intrinsic apoptosis, reliant on BAX and BAK, has been postulated to be fundamental for morphogenesis, but its precise contribution to this process has not been fully explored in mammals. Our structural analysis of BOK suggests close resemblance to BAX and BAK structures. Notably, Bok -/- Bax -/- Bak -/- animals exhibited more severe defects and died earlier than Bax -/- Bak -/- mice, implying that BOK has overlapping roles with BAX and BAK during developmental cell death. By analyzing Bok -/- Bax -/- Bak -/- triple-knockout mice whose cells are incapable of undergoing intrinsic apoptosis, we identified tissues that formed well without this process. We provide evidence that necroptosis, pyroptosis, or autophagy does not substantially substitute for the loss of apoptosis. Albeit very rare, unexpected attainment of adult Bok -/- Bax -/- Bak -/- mice suggests that morphogenesis can proceed entirely without apoptosis mediated by these proteins and possibly without cell death in general.


  • Organizational Affiliation

    The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia; Department of Medical Biology, The University of Melbourne, Melbourne, Victoria 3052, Australia. Electronic address: ke@wehi.edu.au.


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Bcl-2-related ovarian killer protein
A, B
168Gallus gallusMutation(s): 0 
Gene Names: BOK
UniProt
Find proteins for Q9I8I2 (Gallus gallus)
Explore Q9I8I2 
Go to UniProtKB:  Q9I8I2
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupQ9I8I2
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.80 Å
  • R-Value Free: 0.232 
  • R-Value Work: 0.195 
  • R-Value Observed: 0.197 
  • Space Group: P 1 21 1
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 41.559α = 90
b = 59.62β = 96.26
c = 64.083γ = 90
Software Package:
Software NamePurpose
PHENIXrefinement
XDSdata reduction
XDSdata scaling
PHASERphasing

Structure Validation

View Full Validation Report



Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2018-02-28
    Type: Initial release
  • Version 1.1: 2019-03-13
    Changes: Data collection, Database references
  • Version 1.2: 2020-09-09
    Changes: Structure summary
  • Version 1.3: 2023-10-04
    Changes: Data collection, Database references, Refinement description