5WA2

Crystal structure of Toxoplasma gondii SAG3 (SRS57)

PDB DOI: https://doi.org/10.2210/pdb5WA2/pdb

Classification: MEMBRANE PROTEIN
Organism(s): Toxoplasma gondii
Expression System: Trichoplusia ni
Mutation(s): No

Deposited: 2017-06-24 Released: 2018-06-27
Deposition Author(s): Parker, M.L., Boulanger, M.J.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 1.59 Å
R-Value Free: 0.191
R-Value Work: 0.169
R-Value Observed: 0.170

wwPDB Validation 3D Report Full Report

This is version 1.1 of the entry. See complete history.

Literature

A Toxoplasma lectin-specific activity for sulfated proteoglycans thought to promote infection competency is not dependent on TgSRS57 (TgSAG3)

Pszenny, V., Parker, M.L., Ramaswamy, R., Grigg, M.E., Boulanger, M.J.

To be published.

Macromolecule Content

Total Structure Weight: 33.9 kDa
Atom Count: 2,524
Modelled Residue Count: 277
Deposited Residue Count: 304
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Surface antigen	A	304	Toxoplasma gondii	Mutation(s): 0 Gene Names: SAG3
UniProt
Find proteins for Q9BJ39 (Toxoplasma gondii) Explore Q9BJ39 Go to UniProtKB: Q9BJ39
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q9BJ39
Sequence Annotations Expand
Reference Sequence

Small Molecules

Ligands 1 Unique
ID	Chains	Name / Formula / InChI Key	2D Diagram	3D Interactions
GOL Query on GOL Download Ideal Coordinates CCD File SDF format, chain B [auth A] MOL2 format, chain B [auth A]	B [auth A]	GLYCEROL C₃ H₈ O₃ PEDCQBHIVMGVHV-UHFFFAOYSA-N		Interactions Focus chain B [auth A] Interactions & Density Focus chain B [auth A]

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 1.59 Å
R-Value Free: 0.191
R-Value Work: 0.169
R-Value Observed: 0.170
Space Group: P 2₁ 2₁ 2₁

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 48.13	α = 90
b = 59.71	β = 90
c = 101.42	γ = 90

Software Package:

Software Name	Purpose
MOSFLM	data reduction
Aimless	data scaling
PHASER	phasing
PHENIX	refinement
PDB_EXTRACT	data extraction

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2018-06-27

Deposition Author(s):

Boulanger, M.J.

Revision History (Full details and data files)

Version 1.0: 2018-06-27
Type: Initial release
Version 1.1: 2023-10-04
Changes: Data collection, Database references, Refinement description

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.