Download MendeleyPatB1 is an O-acetyltransferase that decorates secondary cell wall polysaccharides.
Sychantha, D., Little, D.J., Chapman, R.N., Boons, G.J., Robinson, H., Howell, P.L., Clarke, A.J.(2018) Nat Chem Biol 14: 79-85
- PubMed: 29083419
- DOI: https://doi.org/10.1038/nchembio.2509
- Primary Citation of Related Structures:
5V8D, 5V8E - PubMed Abstract:
O-Acetylation of the secondary cell wall polysaccharides (SCWP) of the Bacillus cereus group of pathogens, which includes Bacillus anthracis, is essential for the proper attachment of surface-layer (S-layer) proteins to their cell walls. Using a variety of pseudosubstrates and a chemically synthesized analog of SCWP, we report here the identification of PatB1 as a SCWP O-acetyltransferase in Bacillus cereus. Additionally, we report the crystal structure of PatB1, which provides detailed insights into the mechanism of this enzyme and defines a novel subfamily of the SGNH family of esterases and lipases. We propose a model for the O-acetylation of SCWP requiring the translocation of acetyl groups from a cytoplasmic source across the plasma membrane by PatA1 and PatA2 for their transfer to SCWP by PatB1.
Organizational Affiliation:
Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada.
