5UFT

Crystal Structure of a Nitrogen-fixing NifU-like protein (N-terminal) from Brucella abortus

PDB DOI: https://doi.org/10.2210/pdb5UFT/pdb

Classification: METAL BINDING PROTEIN
Organism(s): Brucella abortus 2308
Expression System: Escherichia coli BL21(DE3)
Mutation(s): No

Deposited: 2017-01-05 Released: 2017-01-25
Deposition Author(s): Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.35 Å
R-Value Free: 0.259
R-Value Work: 0.225
R-Value Observed: 0.228

wwPDB Validation 3D Report Full Report

This is version 1.1 of the entry. See complete history.

Literature

Crystal Structure of a Nitrogen-fixing NifU-like protein (N-terminal) from Brucella abortus

Dranow, D.M., Mayclin, S.J., Buchko, G.W., Lorimer, D.D., Edwards, T.E.

To be published.

Macromolecule Content

Total Structure Weight: 16.41 kDa
Atom Count: 1,094
Modelled Residue Count: 144
Deposited Residue Count: 150
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Nitrogen-fixing NifU-like, N-terminal	A	150	Brucella abortus 2308	Mutation(s): 0 Gene Names: BAB1_1096
UniProt
Find proteins for Q2YQ04 (Brucella abortus (strain 2308)) Explore Q2YQ04 Go to UniProtKB: Q2YQ04
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q2YQ04
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.35 Å
R-Value Free: 0.259
R-Value Work: 0.225
R-Value Observed: 0.228
Space Group: P 3₁ 2 1
Diffraction Data: https://doi.org/10.18430/M35UFT

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 42.96	α = 90
b = 42.96	β = 90
c = 149.31	γ = 120

Software Package:

Software Name	Purpose
XSCALE	data scaling
PHENIX	refinement
PDB_EXTRACT	data extraction
XDS	data reduction
MR-Rosetta	phasing

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2017-01-25

Deposition Author(s):

Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Revision History (Full details and data files)

Version 1.0: 2017-01-25
Type: Initial release
Version 1.1: 2023-10-04
Changes: Data collection, Database references, Refinement description

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.