Download MendeleyCrystal structure of a Short chain dehydrogenase from Burkholderia cenocepacia J2315 in complex with NADP
Abendroth, J., Dranow, D.M., Lorimer, D.D., Edwards, T.E.To be published.
5U4S
Crystal structure of a Short chain dehydrogenase from Burkholderia cenocepacia J2315 in complex with NADP.
- PDB DOI: https://doi.org/10.2210/pdb5U4S/pdb
- Classification: OXIDOREDUCTASE
- Organism(s): Burkholderia cenocepacia J2315
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2016-12-06 Released: 2017-01-18
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.40 Å
- R-Value Free: 0.166
- R-Value Work: 0.148
- R-Value Observed: 0.149
This is version 1.1 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Putative short chain dehydrogenase | 245 | Burkholderia cenocepacia J2315 | Mutation(s): 0 Gene Names: BCAM2128 |  | |
UniProt | |||||
Find proteins for B4EFS5 (Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610)) Explore B4EFS5 Go to UniProtKB: B4EFS5 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | B4EFS5 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| NAP Query on NAP | C [auth A], F [auth B] | NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE C21 H28 N7 O17 P3 XJLXINKUBYWONI-NNYOXOHSSA-N |  | ||
| BEZ Query on BEZ | D [auth A], G [auth B] | BENZOIC ACID C7 H6 O2 WPYMKLBDIGXBTP-UHFFFAOYSA-N |  | ||
| PO4 Query on PO4 | E [auth A] | PHOSPHATE ION O4 P NBIIXXVUZAFLBC-UHFFFAOYSA-K |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.40 Å
- R-Value Free: 0.166
- R-Value Work: 0.148
- R-Value Observed: 0.149
- Space Group: P 21 21 21
- Diffraction Data: https://doi.org/10.18430/M35U4S
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 39.27 | α = 90 |
| b = 75.66 | β = 90 |
| c = 146.01 | γ = 90 |
| Software Name | Purpose |
|---|---|
| XDS | data reduction |
| XSCALE | data scaling |
| PHASER | phasing |
| ARP | model building |
| Coot | model building |
| PHENIX | refinement |
| PDB_EXTRACT | data extraction |
Entry History
Deposition Data
- Released Date: 2017-01-18 Deposition Author(s): Seattle Structural Genomics Center for Infectious Disease (SSGCID)
Revision History (Full details and data files)
- Version 1.0: 2017-01-18
Type: Initial release - Version 1.1: 2024-03-06
Changes: Data collection, Database references
