5TI8

Crystal structure of a putrescine aminotransferase from Pseudomonas sp. strain AAC.

(2017) Acta Crystallogr F Struct Biol Commun 73: 29-35

• PubMed: 28045391 Search on PubMedSearch on PubMed Central
• DOI: https://doi.org/10.1107/S2053230X16019658
• Primary Citation of Related Structures:  
  5TI8


• PubMed Abstract: 
  

  The putrescine aminotransferase KES24511 from Pseudomonas sp. strain AAC was previously identified as an industrially relevant enzyme based on the discovery that it is able to promiscuously catalyse the transamination of 12-aminododecanoic acid. Here, the cloning, heterologous expression, purification and successful crystallization of the KES24511 protein are reported, which ultimately generated crystals adopting space group I2. The crystals diffracted X-rays to 2.07 Å resolution and data were collected using the microfocus beamline of the Australian Synchrotron. The structure was solved using molecular replacement, with a monomer from PDB entry 4a6t as the search model.

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Organizational Affiliation: 

Land and Water, CSIRO, GPO Box 1700, Canberra, ACT 2601, Australia.