Download MendeleyCHARACTERISATION OF A FURANOSE SPECIFIC ABC TRANSPORTER ESSENTIAL FOR ARABINOSE UTILISATION FROM THE LIGNOCELLULOSE DEGRADING BACTERIUM SHEWANELLA SP. ANA-3
Herman, R., Drousiotis, K., Wilkinson, A.J., Thomas, G.H.To be published.
5OCP
The periplasmic binding protein component of the arabinose ABC transporter from Shewanella sp. ANA-3 bound to alpha and beta-L-arabinofuranose
- PDB DOI: https://doi.org/10.2210/pdb5OCP/pdb
- Classification: SUGAR BINDING PROTEIN
- Organism(s): Shewanella sp. ANA-3
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2017-07-03 Released: 2018-08-01
- Funding Organization(s): Biotechnology and Biological Sciences Research Council
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.70 Å
- R-Value Free: 0.204
- R-Value Work: 0.167
- R-Value Observed: 0.169
This is version 3.1 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Periplasmic binding protein/LacI transcriptional regulator | 302 | Shewanella sp. ANA-3 | Mutation(s): 0 Gene Names: Shewana3_2073 |  | |
UniProt | |||||
Find proteins for A0KWY4 (Shewanella sp. (strain ANA-3)) Explore A0KWY4 Go to UniProtKB: A0KWY4 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | A0KWY4 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 4 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| FUB (Subject of Investigation/LOI) Query on FUB | J [auth A], O [auth B] | beta-L-arabinofuranose C5 H10 O5 HMFHBZSHGGEWLO-KLVWXMOXSA-N |  | ||
| AHR (Subject of Investigation/LOI) Query on AHR | I [auth A], N [auth B] | alpha-L-arabinofuranose C5 H10 O5 HMFHBZSHGGEWLO-QMKXCQHVSA-N |  | ||
| GOL Query on GOL | C [auth A] D [auth A] E [auth A] F [auth A] G [auth A] | GLYCEROL C3 H8 O3 PEDCQBHIVMGVHV-UHFFFAOYSA-N |  | ||
| ACT Query on ACT | M [auth B] | ACETATE ION C2 H3 O2 QTBSBXVTEAMEQO-UHFFFAOYSA-M |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.70 Å
- R-Value Free: 0.204
- R-Value Work: 0.167
- R-Value Observed: 0.169
- Space Group: P 21 21 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 73.917 | α = 90 |
| b = 86.327 | β = 90 |
| c = 87.282 | γ = 90 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| Aimless | data scaling |
| PDB_EXTRACT | data extraction |
| MOLREP | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2018-08-01 Deposition Author(s): Herman, R.
| Funding Organization | Location | Grant Number |
|---|---|---|
| Biotechnology and Biological Sciences Research Council | United Kingdom | BB/N01040X/1 |
Revision History (Full details and data files)
- Version 1.0: 2018-08-01
Type: Initial release - Version 2.0: 2018-12-19
Changes: Atomic model, Data collection, Derived calculations - Version 3.0: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Atomic model, Data collection, Derived calculations, Structure summary - Version 3.1: 2024-01-17
Changes: Data collection, Database references, Refinement description, Structure summary
