5JYJ

Crystal structure of mouse JUNO

PDB DOI: https://doi.org/10.2210/pdb5JYJ/pdb

Classification: CELL ADHESION
Organism(s): Mus musculus
Expression System: Spodoptera frugiperda
Mutation(s): Yes

Deposited: 2016-05-14 Released: 2017-05-24
Deposition Author(s): Kato, K., Nishimasu, H., Morita, J., Ishitani, R., Nureki, O.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.30 Å
R-Value Free: 0.233
R-Value Work: 0.208
R-Value Observed: 0.209

wwPDB Validation 3D Report Full Report

This is version 2.0 of the entry. See complete history.

Literature

Crystal structure of the egg IZUMO1 receptor JUNO

Kato, K., Satouh, Y., Nishimasu, H., Kurabayashi, A., Morita, J., Fujihara, Y., Oji, A., Ishitani, R., Ikawa, M., Nureki, O.

To be published.

Macromolecule Content

Total Structure Weight: 24.91 kDa
Atom Count: 1,643
Modelled Residue Count: 197
Deposited Residue Count: 210
Unique protein chains: 1

Macromolecules

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(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Sperm-egg fusion protein Juno	A	210	Mus musculus	Mutation(s): 1 Gene Names: Izumo1r, Folbp3, Folr4, Juno
UniProt & NIH Common Fund Data Resources
Find proteins for Q9EQF4 (Mus musculus) Explore Q9EQF4 Go to UniProtKB: Q9EQF4
IMPC: MGI:1929185
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q9EQF4
Sequence Annotations Expand
Reference Sequence

Oligosaccharides

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Entity ID: 2
Molecule	Chains	Length	2D Diagram	Glycosylation	3D Interactions
2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	B	2		N-Glycosylation	Interactions Focus chain B Interactions & Density Focus chain B
Glycosylation Resources
GlyTouCan: G42666HT GlyCosmos: G42666HT GlyGen: G42666HT

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.30 Å
R-Value Free: 0.233
R-Value Work: 0.208
R-Value Observed: 0.209
Space Group: P 2 2₁ 2₁

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 42.396	α = 90
b = 54.449	β = 90
c = 87.145	γ = 90

Software Package:

Software Name	Purpose
PHENIX	refinement
XDS	data reduction
XDS	data scaling
MOLREP	phasing

Structure Validation

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Entry History

Deposition Data

Released Date: 2017-05-24

Deposition Author(s):

Revision History (Full details and data files)

Version 1.0: 2017-05-24
Type: Initial release
Version 2.0: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Atomic model, Data collection, Derived calculations, Structure summary

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Deposit Data

Help and Resources

5JYJ

Crystal structure of mouse JUNO

Crystal structure of the egg IZUMO1 receptor JUNO

Entity ID: 1

UniProt & NIH Common Fund Data Resources

Entity Groups

Sequence Annotations

Expand

Entity ID: 2

Glycosylation Resources

Experimental Data

Structure Validation

Deposition Data

Revision History (Full details and data files)