Download MendeleySortase B from Listeria monocytogenes.
Osipiuk, J., Zhou, M., Grimshaw, S., Anderson, W.F., Joachimiak, A., Center for Structural Genomics of Infectious Diseases (CSGID)To be published.
5JCV
Sortase B from Listeria monocytogenes.
- PDB DOI: https://doi.org/10.2210/pdb5JCV/pdb
- Classification: HYDROLASE
- Organism(s): Listeria monocytogenes EGD-e
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2016-04-15 Released: 2016-04-27
- Funding Organization(s): National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.23 Å
- R-Value Free: 0.249
- R-Value Work: 0.207
- R-Value Observed: 0.209
wwPDB Validation 3D Report Full Report
This is version 1.2 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Lmo2181 protein | 183 | Listeria monocytogenes EGD-e | Mutation(s): 0 Gene Names: lmo2181 |  | |
UniProt | |||||
Find proteins for Q8Y588 (Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)) Explore Q8Y588 Go to UniProtKB: Q8Y588 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q8Y588 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| SO4 Query on SO4 | B [auth A] | SULFATE ION O4 S QAOWNCQODCNURD-UHFFFAOYSA-L |  | ||
| CL Query on CL | C [auth A] | CHLORIDE ION Cl VEXZGXHMUGYJMC-UHFFFAOYSA-M |  | ||
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| MSE Query on MSE | A | L-PEPTIDE LINKING | C5 H11 N O2 Se |  | MET |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.23 Å
- R-Value Free: 0.249
- R-Value Work: 0.207
- R-Value Observed: 0.209
- Space Group: I 41 2 2
- Diffraction Data: https://doi.org/10.18430/M35JCV
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 70.155 | α = 90 |
| b = 70.155 | β = 90 |
| c = 224.471 | γ = 90 |
| Software Name | Purpose |
|---|---|
| SBC-Collect | data collection |
| REFMAC | refinement |
| PHENIX | refinement |
| PDB_EXTRACT | data extraction |
| HKL-3000 | data reduction |
| HKL-3000 | data scaling |
| HKL-3000 | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2016-04-27 Deposition Author(s): Osipiuk, J., Zhou, M., Grimshaw, S., Anderson, W.F., Joachimiak, A., Center for Structural Genomics of Infectious Diseases (CSGID)
| Funding Organization | Location | Grant Number |
|---|---|---|
| National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) | United States | HHSN272200700058C |
| National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) | United States | HHSN272201200026C |
Revision History (Full details and data files)
- Version 1.0: 2016-04-27
Type: Initial release - Version 1.1: 2017-09-20
Changes: Author supporting evidence, Derived calculations, Refinement description - Version 1.2: 2019-12-11
Changes: Author supporting evidence
