Download MendeleyStructure of the archael ExoIII homologue Mm3148 at 1.4 Angstrom
Lakomek, K., Dickmanns, A., Ber, S., Fritz, H.-J., Ficner, R.To be published.
5J8N
Exonuclease III homologue Mm3148 from Methanosarcina mazei
- PDB DOI: https://doi.org/10.2210/pdb5J8N/pdb
- Classification: HYDROLASE
- Organism(s): Methanosarcina mazei Go1
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): Yes
- Deposited: 2016-04-08 Released: 2016-04-27
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.44 Å
- R-Value Free: 0.171
- R-Value Work: 0.147
- R-Value Observed: 0.148
wwPDB Validation 3D Report Full Report
This is version 1.2 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Exodeoxyribonuclease III | 258 | Methanosarcina mazei Go1 | Mutation(s): 3 Gene Names: MM_3148 EC: 3.1.11.2 |  | |
UniProt | |||||
Find proteins for Q8PSD1 (Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88)) Explore Q8PSD1 Go to UniProtKB: Q8PSD1 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q8PSD1 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| PG4 Query on PG4 | C [auth A] | TETRAETHYLENE GLYCOL C8 H18 O5 UWHCKJMYHZGTIT-UHFFFAOYSA-N |  | ||
| MG Query on MG | B [auth A] | MAGNESIUM ION Mg JLVVSXFLKOJNIY-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.44 Å
- R-Value Free: 0.171
- R-Value Work: 0.147
- R-Value Observed: 0.148
- Space Group: P 21 21 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 46.726 | α = 90 |
| b = 64.564 | β = 90 |
| c = 84.89 | γ = 90 |
| Software Name | Purpose |
|---|---|
| PHENIX | refinement |
| SCALEPACK | data scaling |
| MOLREP | phasing |
| PDB_EXTRACT | data extraction |
Entry History
Deposition Data
- Released Date: 2016-04-27 Deposition Author(s): Lakomek, K., Dickmanns, A., Ficner, R.
Revision History (Full details and data files)
- Version 1.0: 2016-04-27
Type: Initial release - Version 1.1: 2019-07-17
Changes: Data collection - Version 1.2: 2024-01-10
Changes: Data collection, Database references, Derived calculations, Refinement description
