5IMU

A fragment of conserved hypothetical protein Rv3899c (residues 184-410) from Mycobacterium tuberculosis

PDB DOI: https://doi.org/10.2210/pdb5IMU/pdb

Classification: SIGNALING PROTEIN
Organism(s): Mycobacterium tuberculosis H37Rv
Expression System: Escherichia coli BL21(DE3)
Mutation(s): No

Deposited: 2016-03-07 Released: 2017-03-08
Deposition Author(s): Li, D.F., Gao, Y.R., Liu, Y.Y., Bi, L.J.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 1.90 Å
R-Value Free: 0.190
R-Value Work: 0.160
R-Value Observed: 0.161

wwPDB Validation 3D Report Full Report

This is version 1.1 of the entry. See complete history.

Literature

Crystal structure of Rv3899c(184-410), a hypothetical protein from Mycobacterium tuberculosis

Liu, Y.Y., Gao, Y.R., Li, D.F., Fleming, J., Li, H.L., Bi, L.J.

(2016) Acta Crystallogr F Struct Biol Commun 72: 642-645

PubMed: 27487929 Search on PubMedSearch on PubMed Central
DOI: https://doi.org/10.1107/S2053230X16010943
Primary Citation of Related Structures:
5IMU

PubMed Abstract:
Rv3899c is a hypothetical protein from Mycobacterium tuberculosis which is conserved across mycobacteria. It is predicted to be secreted and has been found in culture filtrates. It has been proposed as a potential vaccine candidate; however, its biological function is unknown. Here, the global structure of Rv3899c(184-410), a fragment of Rv3899c, is reported. The structure resembles the shell of a sea snail, and its N- and C-termini form two relatively independent compact domains: an α/β/α sandwich folding domain and an α-helix bundle domain. There are no reported protein structures for any Rv3899c homologues; this structure provides the first structural glimpse of a new protein family consisting of Rv3899c and its homologues.

Organizational Affiliation

Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People's Republic of China.

Macromolecule Content

Total Structure Weight: 24.29 kDa
Atom Count: 1,995
Modelled Residue Count: 217
Deposited Residue Count: 227
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Tat (Twin-arginine translocation) pathway signal sequence containing protein	A	227	Mycobacterium tuberculosis H37Rv	Mutation(s): 0 Gene Names: Rv3899c, LH57_21230
UniProt
Find proteins for O05446 (Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)) Explore O05446 Go to UniProtKB: O05446
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	O05446
Sequence Annotations Expand
Reference Sequence

Small Molecules

Ligands 1 Unique
ID	Chains	Name / Formula / InChI Key	2D Diagram	3D Interactions
K Query on K Download Ideal Coordinates CCD File SDF format, chain B [auth A] MOL2 format, chain B [auth A]	B [auth A]	POTASSIUM ION K NPYPAHLBTDXSSS-UHFFFAOYSA-N		Interactions Focus chain B [auth A] Interactions & Density Focus chain B [auth A]