5HLJ

Crystal Structure of Major Envelope Protein VP24 from White Spot Syndrome Virus


Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 2.41 Å
  • R-Value Free: 0.193 
  • R-Value Work: 0.171 
  • R-Value Observed: 0.173 

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This is version 1.2 of the entry. See complete history


Literature

Crystal Structure of Major Envelope Protein VP24 from White Spot Syndrome Virus

Sun, L.F.Su, Y.T.Zhao, Y.H.Fu, Z.Q.Wu, Y.K.

(2016) Sci Rep 6: 32309-32309

  • DOI: https://doi.org/10.1038/srep32309
  • Primary Citation of Related Structures:  
    5HLJ

  • PubMed Abstract: 

    White spot syndrome virus (WSSV) is one of the major and most serious pathogen in the shrimp industry. As one of the most abundant envelope protein, VP24 acts as a core protein interacting with other structure proteins and plays an important role in virus assembly and infection. Here, we have presented the crystal structure of VP24 from WSSV. In the structure, VP24 consists of a nine-stranded β-barrel fold with mostly antiparallel β-strands, and the loops extending out the β-barrel at both N-terminus and C-terminus, which is distinct to those of the other two major envelope proteins VP28 and VP26. Structural comparison of VP24 with VP26 and VP28 reveals opposite electrostatic surface potential properties of them. These structural differences could provide insight into their differential functional mechanisms and roles for virus assembly and infection. Moreover, the structure reveals a trimeric assembly, suggesting a likely natural conformation of VP24 in viral envelope. Therefore, in addition to confirming the evolutionary relationship among the three abundant envelope proteins of WSSV, our structural studies also facilitate a better understanding of the molecular mechanism underlying special roles of VP24 in WSSV assembly and infection.


  • Organizational Affiliation

    State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, China.


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
VP24188White spot syndrome virusMutation(s): 0 
UniProt
Find proteins for Q80BF2 (White spot syndrome virus)
Explore Q80BF2 
Go to UniProtKB:  Q80BF2
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupQ80BF2
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 2.41 Å
  • R-Value Free: 0.193 
  • R-Value Work: 0.171 
  • R-Value Observed: 0.173 
  • Space Group: I 21 3
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 139.735α = 90
b = 139.735β = 90
c = 139.735γ = 90
Software Package:
Software NamePurpose
PHENIXrefinement
HKL-2000data scaling
PDB_EXTRACTdata extraction
SCALEPACKdata reduction
PHENIXphasing

Structure Validation

View Full Validation Report



Entry History & Funding Information

Deposition Data


Funding OrganizationLocationGrant Number
National Natural Science Foundation of ChinaChina31302225

Revision History  (Full details and data files)

  • Version 1.0: 2016-09-14
    Type: Initial release
  • Version 1.1: 2017-10-18
    Changes: Author supporting evidence, Derived calculations
  • Version 1.2: 2024-03-20
    Changes: Data collection, Database references