5GLE

The structure of Vibrio anguillarum775 AngB-ICL

PDB DOI: https://doi.org/10.2210/pdb5GLE/pdb

Classification: LYASE
Organism(s): Vibrio anguillarum 775
Expression System: Escherichia coli BL21(DE3)
Mutation(s): Yes

Deposited: 2016-07-10 Released: 2017-10-25
Deposition Author(s): Du, J.S., Ma, Q.G.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.10 Å
R-Value Free: 0.230
R-Value Work: 0.206
R-Value Observed: 0.207

wwPDB Validation 3D Report Full Report

This is version 1.1 of the entry. See complete history.

Literature

The structure of Vibrio anguillarum775 AngB-ICL protein

Du, J.S., Deng, T., Ma, Q.G.

To be published.

Macromolecule Content

Total Structure Weight: 46.83 kDa
Atom Count: 3,491
Modelled Residue Count: 416
Deposited Residue Count: 416
Unique protein chains: 2

Macromolecules

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(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Ischorismate lyase	A	208	Vibrio anguillarum 775	Mutation(s): 0 Gene Names: G, angB
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
Sequence Annotations Expand
Reference Sequence

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 2
Molecule	Chains	Sequence Length	Organism	Details	Image
Ischorismate lyase	B	208	Vibrio anguillarum 775	Mutation(s): 1 Gene Names: G, angB
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.10 Å
R-Value Free: 0.230
R-Value Work: 0.206
R-Value Observed: 0.207
Space Group: P 3₁ 2 1

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 140.19	α = 90
b = 140.19	β = 90
c = 59.3	γ = 120

Software Package:

Software Name	Purpose
PHENIX	refinement
HKL-2000	data processing
PHENIX	refinement

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2017-10-25

Deposition Author(s):

Revision History (Full details and data files)

Version 1.0: 2017-10-25
Type: Initial release
Version 1.1: 2023-11-08
Changes: Data collection, Database references, Refinement description

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.