5AJH

Crystal structure of Fusarium oxysporum cutinase


Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.90 Å
  • R-Value Free: 0.224 
  • R-Value Work: 0.181 
  • R-Value Observed: 0.184 

wwPDB Validation   3D Report Full Report


This is version 1.2 of the entry. See complete history


Literature

Structural and Functional Studies of a Fusarium Oxysporum Cutinase with Polyethylene Terephthalate Modification Potential.

Dimarogona, M.Nikolaivits, E.Kanelli, M.Christakopoulos, P.Sandgren, M.Topakas, E.

(2015) Biochim Biophys Acta 1850: 2308

  • DOI: https://doi.org/10.1016/j.bbagen.2015.08.009
  • Primary Citation of Related Structures:  
    5AJH

  • PubMed Abstract: 

    Cutinases are serine hydrolases that degrade cutin, a polyester of fatty acids that is the main component of plant cuticle. These biocatalysts have recently attracted increased biotechnological interest due to their potential to modify and degrade polyethylene terephthalate (PET), as well as other synthetic polymers.


  • Organizational Affiliation

    Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str., Zografou Campus, Athens 15780, Greece; Department of Chemistry and Biotechnology, Swedish University of Agricultural Science, SE-75007 Uppsala, Sweden.


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
CUTINASE
A, B, C
226Fusarium oxysporum f. sp. raphani 54005Mutation(s): 0 
EC: 3.1.1.74
Membrane Entity: Yes 
UniProt
Find proteins for X0BTD8 (Fusarium oxysporum f. sp. raphani 54005)
Explore X0BTD8 
Go to UniProtKB:  X0BTD8
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupX0BTD8
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.90 Å
  • R-Value Free: 0.224 
  • R-Value Work: 0.181 
  • R-Value Observed: 0.184 
  • Space Group: P 21 21 21
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 35.906α = 90
b = 60.141β = 90
c = 241.965γ = 90
Software Package:
Software NamePurpose
REFMACrefinement
XDSdata reduction
Aimlessdata scaling
PHASERphasing

Structure Validation

View Full Validation Report



Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2015-09-02
    Type: Initial release
  • Version 1.1: 2015-10-07
    Changes: Database references
  • Version 1.2: 2024-01-10
    Changes: Data collection, Database references, Derived calculations, Other, Refinement description