Download MendeleyStructural Analysis and Knock-Out of a Burkholderia Pseudomallei Homolog of the Eukaryotic Transcription Coactivator Pc4.
Werten, S., Kohler, C., Bayer, N., Steinmetz, I., Hinrichs, W.(2016) Gene 557: 140
- PubMed: 26625975
- DOI: https://doi.org/10.1016/j.gene.2015.11.037
- Primary Citation of Related Structures:
5A4N, 5A4O - PubMed Abstract:
Homologs of the eukaryotic transcription coactivator PC4, which also functions in DNA repair and oxidative stress, were recently identified in prokaryotes. Crystallographic analysis of BPSL1147, a putative homolog from the pathogen Burkholderia pseudomallei K96243, reveals a highly conserved core structure and suggests a nucleic acid binding mode similar to that of PC4. Knock-out and complementation experiments do not reveal distinguishing phenotypes under normal growth conditions or in the presence of H2O2, arguing against a critical role in repair or the oxidative stress response of Burkholderia. These results may reflect redundancy or point at a bacteriophage origin of Burkholderia PC4 homologs.
Organizational Affiliation:
Department of Molecular Structural Biology, Institute for Biochemistry, University of Greifswald, Felix-Hausdorff-Strasse 4, D-17487 Greifswald, Germany. Electronic address: sebastiaan.werten@uni-greifswald.de.
