5A2T

The Molecular Basis for Flexibility in the Flexible Filamentous Plant Viruses

PDB DOI: https://doi.org/10.2210/pdb5A2T/pdb
NAKB: 5A2T
EM Map EMD-3020: EMDB EMDataResource

Classification: VIRAL PROTEIN
Organism(s): Bamboo mosaic virus
Mutation(s): No

Deposited: 2015-05-23 Released: 2015-07-22
Deposition Author(s): DiMaio, F., Chen, C.C., Yu, X., Frenz, B., Hsu, Y.H., Lin, N.S., Egelman, E.H.

Experimental Data Snapshot

Method: ELECTRON MICROSCOPY
Resolution: 5.60 Å
Aggregation State: FILAMENT
Reconstruction Method: HELICAL

wwPDB Validation 3D Report Full Report

This is version 1.1 of the entry. See complete history.

Literature

The Molecular Basis for Flexibility in the Flexible Filamentous Plant Viruses.

Dimaio, F., Chen, C., Yu, X., Frenz, B., Hsu, Y., Lin, N., Egelman, E.H.

(2015) Nat Struct Mol Biol 22: 642

PubMed: 26167882 Search on PubMedSearch on PubMed Central
DOI: https://doi.org/10.1038/nsmb.3054
Primary Citation of Related Structures:
5A2T

PubMed Abstract:
Flexible filamentous plant viruses cause more than half the viral crop damage in the world but are also potentially useful for biotechnology. Structural studies began more than 75 years ago but have failed, owing to the virion's extreme flexibility. We have used cryo-EM to generate an atomic model for bamboo mosaic virus, which reveals flexible N- and C-terminal extensions that allow deformation while still maintaining structural integrity.

Organizational Affiliation

Department of Biochemistry, University of Washington, Seattle, Washington, USA.

Macromolecule Content

Total Structure Weight: 597.01 kDa
Atom Count: 41,925
Modelled Residue Count: 5,225
Deposited Residue Count: 5,225
Unique protein chains: 1
Unique nucleic acid chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
COAT PROTEIN	A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y	204	Bamboo mosaic virus	Mutation(s): 0
UniProt
Find proteins for O37178 (Bamboo mosaic virus) Explore O37178 Go to UniProtKB: O37178
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	O37178
Sequence Annotations Expand
Reference Sequence

Find similar nucleic acids by:

(by identity cutoff) | 3D Structure

Entity ID: 2
Molecule	Chains	Length	Organism	Image
BAMBOO MOSAIC VIRUS	Z	125	Bamboo mosaic virus
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: ELECTRON MICROSCOPY
Resolution: 5.60 Å
Aggregation State: FILAMENT
Reconstruction Method: HELICAL

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2015-07-22

Deposition Author(s):

Revision History (Full details and data files)

Version 1.0: 2015-07-22
Type: Initial release
Version 1.1: 2015-08-19
Changes: Database references

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.