Download MendeleyStructural insights into the molecular mechanism of MERS Coronavirus RNA ribose 2'-O-methylation by nsp16/nsp10 protein complex
Wei, S.M., Yang, L., Ke, Z.H., Liu, Q.Y., Yang, Z.Z., Chen, Y., Guo, D.Y., Fan, C.P.To be published.
5YN5
Crystal structure of MERS-CoV nsp10/nsp16 complex
- PDB DOI: https://doi.org/10.2210/pdb5YN5/pdb
- Classification: TRANSFERASE
- Organism(s): Human betacoronavirus 2c EMC/2012
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2017-10-24 Released: 2018-12-05
- Funding Organization(s): NSFC
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.96 Å
- R-Value Free: 0.214
- R-Value Work: 0.181
- R-Value Observed: 0.182
wwPDB Validation 3D Report Full Report
This is version 1.1 of the entry. See complete history.
Literature
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| nsp16 protein | 303 | Human betacoronavirus 2c EMC/2012 | Mutation(s): 0 Gene Names: orf1ab |  | |
UniProt | |||||
Find proteins for K0BWD0 (Human betacoronavirus 2c EMC/2012) Explore K0BWD0 Go to UniProtKB: K0BWD0 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | K0BWD0 | ||||
Sequence AnnotationsExpand | |||||
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Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| nsp10 protein | 140 | Human betacoronavirus 2c EMC/2012 | Mutation(s): 0 Gene Names: orf1ab |  | |
UniProt | |||||
Find proteins for K4LC41 (Human betacoronavirus 2c EMC/2012) Explore K4LC41 Go to UniProtKB: K4LC41 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | K4LC41 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| ZN Query on ZN | C [auth B], D [auth B] | ZINC ION Zn PTFCDOFLOPIGGS-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.96 Å
- R-Value Free: 0.214
- R-Value Work: 0.181
- R-Value Observed: 0.182
- Space Group: P 21 2 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 66.754 | α = 90 |
| b = 70.527 | β = 90 |
| c = 119.254 | γ = 90 |
| Software Name | Purpose |
|---|---|
| PHENIX | refinement |
| XDS | data reduction |
| XSCALE | data scaling |
| PHENIX | phasing |
Entry History & Funding Information
Deposition Data
| Funding Organization | Location | Grant Number |
|---|---|---|
| NSFC | China | 31570762 |
Revision History (Full details and data files)
- Version 1.0: 2018-12-05
Type: Initial release - Version 1.1: 2023-11-22
Changes: Data collection, Database references, Refinement description
