5OFU
Crystal structure of Leishmania major fructose-1,6-bisphosphatase in T-state.
- PDB DOI: https://doi.org/10.2210/pdb5OFU/pdb
- Classification: TRANSFERASE
- Organism(s): Leishmania major
- Expression System: Escherichia coli 'BL21-Gold(DE3)pLysS AG
- Mutation(s): No
- Deposited: 2017-07-11 Released: 2017-09-20
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.62 Å
- R-Value Free: 0.216
- R-Value Work: 0.189
- R-Value Observed: 0.190
This is version 1.3 of the entry. See complete history.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| FBP protein | 351 | Leishmania major | Mutation(s): 0 Gene Names: FBP, LMJF_04_1160 EC: 3.1.3.11 |  | |
UniProt | |||||
Find proteins for O97193 (Leishmania major) Explore O97193 Go to UniProtKB: O97193 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | O97193 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| AMP Query on AMP | E [auth A], H [auth B], J [auth C], M [auth D] | ADENOSINE MONOPHOSPHATE C10 H14 N5 O7 P UDMBCSSLTHHNCD-KQYNXXCUSA-N |  | ||
| F6P Query on F6P | F [auth A], I [auth B], K [auth C], N [auth D] | 6-O-phosphono-beta-D-fructofuranose C6 H13 O9 P BGWGXPAPYGQALX-ARQDHWQXSA-N |  | ||
| CL Query on CL | G [auth A], L [auth C] | CHLORIDE ION Cl VEXZGXHMUGYJMC-UHFFFAOYSA-M |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.62 Å
- R-Value Free: 0.216
- R-Value Work: 0.189
- R-Value Observed: 0.190
- Space Group: P 21 21 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 90.59 | α = 90 |
| b = 109.25 | β = 90 |
| c = 140.51 | γ = 90 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| xia2 | data reduction |
| Aimless | data scaling |
| PHASER | phasing |
Entry History
Deposition Data
- Released Date: 2017-09-20 Deposition Author(s): Yuan, M., Vasquez-Valdivieso, M.G., McNae, I.W., Michels, P.A.M., Fothergill-Gilmore, L.A., Walkinshaw, M.D.
Revision History (Full details and data files)
- Version 1.0: 2017-09-20
Type: Initial release - Version 1.1: 2017-10-18
Changes: Database references - Version 1.2: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Data collection, Derived calculations, Structure summary - Version 1.3: 2024-01-17
Changes: Data collection, Database references, Refinement description, Structure summary
