Download MendeleyAtomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED.
de la Cruz, M.J., Hattne, J., Shi, D., Seidler, P., Rodriguez, J., Reyes, F.E., Sawaya, M.R., Cascio, D., Weiss, S.C., Kim, S.K., Hinck, C.S., Hinck, A.P., Calero, G., Eisenberg, D., Gonen, T.(2017) Nat Methods 14: 399-402
- PubMed: 28192420
- DOI: https://doi.org/10.1038/nmeth.4178
- Primary Citation of Related Structures:
5K7N, 5K7O, 5K7P, 5K7Q, 5K7R, 5K7S, 5K7T, 5TY4 - PubMed Abstract:
Traditionally, crystallographic analysis of macromolecules has depended on large, well-ordered crystals, which often require significant effort to obtain. Even sizable crystals sometimes suffer from pathologies that render them inappropriate for high-resolution structure determination. Here we show that fragmentation of large, imperfect crystals into microcrystals or nanocrystals can provide a simple path for high-resolution structure determination by the cryoEM method MicroED and potentially by serial femtosecond crystallography.
Organizational Affiliation:
Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, Virginia, USA.
