5IHJ
Fusion of Maltose-binding Protein and PilA from Acinetobacter baumannii BIDMC57
- PDB DOI: https://doi.org/10.2210/pdb5IHJ/pdb
- Classification: CELL ADHESION
- Organism(s): Escherichia coli K-12, Acinetobacter sp. 216872
- Expression System: Escherichia coli BL21
- Mutation(s): Yes
- Deposited: 2016-02-29 Released: 2016-09-21
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.20 Å
- R-Value Free: 0.244
- R-Value Work: 0.211
wwPDB Validation 3D Report Full Report
This is version 2.0 of the entry. See complete history.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Maltose-binding periplasmic protein,Fimbrial protein | 495 | Escherichia coli K-12, Acinetobacter sp. 216872 This entity is chimeric | Mutation(s): 10 Gene Names: malE, b4034, JW3994, tfpA, J633_2062 |  | |
UniProt | |||||
Find proteins for P0AEX9 (Escherichia coli (strain K12)) Explore P0AEX9 Go to UniProtKB: P0AEX9 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P0AEX9 | ||||
Sequence AnnotationsExpand | |||||
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Oligosaccharides
Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| MPD Query on MPD | C [auth A] | (4S)-2-METHYL-2,4-PENTANEDIOL C6 H14 O2 SVTBMSDMJJWYQN-YFKPBYRVSA-N |  | ||
| GOL Query on GOL | D [auth A], E [auth A], F [auth A], G [auth A] | GLYCEROL C3 H8 O3 PEDCQBHIVMGVHV-UHFFFAOYSA-N |  | ||
| CA Query on CA | H [auth A], I [auth A], J [auth A] | CALCIUM ION Ca BHPQYMZQTOCNFJ-UHFFFAOYSA-N |  | ||
Biologically Interesting Molecules (External Reference) 1 Unique
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| ID | Chains | Name | Type/Class | 2D Diagram | 3D Interactions |
| PRD_900009 Query on PRD_900009 | B | alpha-maltotriose | Oligosaccharide / Nutrient |  | |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.20 Å
- R-Value Free: 0.244
- R-Value Work: 0.211
- Space Group: C 1 2 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 175.07 | α = 90 |
| b = 56.636 | β = 91.6 |
| c = 49.997 | γ = 90 |
| Software Name | Purpose |
|---|---|
| HKL-2000 | data collection |
| HKL-2000 | data scaling |
| PHASER | phasing |
| REFMAC | refinement |
| PDB_EXTRACT | data extraction |
| HKL-2000 | data reduction |
Entry History
Deposition Data
- Released Date: 2016-09-21 Deposition Author(s): Piepenbrink, K.H., Sundberg, E.J.
Revision History (Full details and data files)
- Version 1.0: 2016-09-21
Type: Initial release - Version 1.1: 2016-09-28
Changes: Database references - Version 1.2: 2016-11-09
Changes: Database references - Version 2.0: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Atomic model, Data collection, Database references, Derived calculations, Non-polymer description, Structure summary
