Download MendeleyCrystal structure of M. tuberculosis MazF-mt3 T52D-F62D mutant in complex with 8-mer RNA
Yen, T.J., Brennan, R.G.To be published.
5HKC
Crystal structure of M. tuberculosis MazF-mt3 T52D-F62D mutant in complex with 8-mer RNA
- PDB DOI: https://doi.org/10.2210/pdb5HKC/pdb
- NAKB: 5HKC
- Classification: HYDROLASE/RNA
- Organism(s): Mycobacterium tuberculosis CDC1551, Mycobacterium tuberculosis
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2016-01-14 Released: 2017-01-18
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.68 Å
- R-Value Free: 0.232
- R-Value Work: 0.201
- R-Value Observed: 0.203
wwPDB Validation 3D Report Full Report
This is version 1.2 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Endoribonuclease MazF6 | 114 | Mycobacterium tuberculosis CDC1551 | Mutation(s): 2 Gene Names: mazF6, MT2046 EC: 3.1.27 |  | |
UniProt | |||||
Find proteins for P9WII3 (Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)) Explore P9WII3 Go to UniProtKB: P9WII3 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P9WII3 | ||||
Sequence AnnotationsExpand | |||||
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Find similar nucleic acids by: Sequence | 3D Structure
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Length | Organism | Image | |
| RNA (5'-R(*GP*UP*C)-D(P*U)-R(P*CP*CP*UP*A)-3') | 8 | Mycobacterium tuberculosis |  | ||
Sequence AnnotationsExpand | |||||
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Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.68 Å
- R-Value Free: 0.232
- R-Value Work: 0.201
- R-Value Observed: 0.203
- Space Group: P 1 2 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 48.269 | α = 90 |
| b = 38.798 | β = 107.3 |
| c = 69.178 | γ = 90 |
| Software Name | Purpose |
|---|---|
| HKL-2000 | data scaling |
| PHENIX | refinement |
| PDB_EXTRACT | data extraction |
| HKL-2000 | data reduction |
| PHASER | phasing |
Entry History
Deposition Data
- Released Date: 2017-01-18 Deposition Author(s): Yen, T.J., Brennan, R.G.
Revision History (Full details and data files)
- Version 1.0: 2017-01-18
Type: Initial release - Version 1.1: 2024-03-06
Changes: Data collection, Database references, Refinement description - Version 1.2: 2024-04-03
Changes: Refinement description
