5HJZ

Structure of M. tuberculosis MazF-mt1 (Rv2801c) in complex with RNA

PDB DOI: https://doi.org/10.2210/pdb5HJZ/pdb
NAKB: 5HJZ

Classification: hydrolase/rna
Organism(s): Mycobacterium tuberculosis H37Rv, Mycobacterium tuberculosis
Expression System: Escherichia coli
Mutation(s): No

Deposited: 2016-01-13 Released: 2017-01-18
Deposition Author(s): Yen, T.J., Brennan, R.G.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 1.98 Å
R-Value Free: 0.230
R-Value Work: 0.206
R-Value Observed: 0.208

wwPDB Validation 3D Report Full Report

This is version 1.2 of the entry. See complete history.

Literature

Structure of M. tuberculosis MazF-mt1 (Rv2801c) in complex with RNA

Yen, T.J., Brennan, R.G.

To be published.

Macromolecule Content

Total Structure Weight: 29.36 kDa
Atom Count: 1,983
Modelled Residue Count: 234
Deposited Residue Count: 249
Unique protein chains: 1
Unique nucleic acid chains: 1

Macromolecules

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(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Endoribonuclease MazF9	A, B	119	Mycobacterium tuberculosis H37Rv	Mutation(s): 0 Gene Names: mazF9, mazF-mt1, Rv2801c EC: 3.1
UniProt
Find proteins for P71650 (Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)) Explore P71650 Go to UniProtKB: P71650
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P71650
Sequence Annotations Expand
Reference Sequence

Find similar nucleic acids by: Sequence | 3D Structure

Entity ID: 2
Molecule	Chains	Length	Organism	Image
RNA (5'-R(CPAPUPC)-D(PU)-R(PAPCPCPUPGP*A)-3')	C	11	Mycobacterium tuberculosis
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 1.98 Å
R-Value Free: 0.230
R-Value Work: 0.206
R-Value Observed: 0.208
Space Group: P 2₁ 2₁ 2₁

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 38.799	α = 90
b = 68.542	β = 90
c = 80.162	γ = 90

Software Package:

Software Name	Purpose
PHENIX	refinement
HKL-2000	data scaling
PDB_EXTRACT	data extraction
HKL-2000	data reduction
PHASER	phasing

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2017-01-18

Deposition Author(s):

Revision History (Full details and data files)

Version 1.0: 2017-01-18
Type: Initial release
Version 1.1: 2024-03-06
Changes: Data collection, Database references
Version 1.2: 2024-04-03
Changes: Refinement description

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.