Download MendeleyCrystal structure of CdiI from Burkholderia dolosa AUO158
Michalska, K., Stols, L., Eschenfeldt, W., Goulding, C.W., Joachimiak, A., Midwest Center for Structural Genomics (MCSG)To be published.
5FFP
Crystal structure of CdiI from Burkholderia dolosa AUO158
- PDB DOI: https://doi.org/10.2210/pdb5FFP/pdb
- Classification: ANTITOXIN
- Organism(s): Burkholderia dolosa AU0158
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2015-12-18 Released: 2016-01-20
- Funding Organization(s): National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.50 Å
- R-Value Free: 0.205
- R-Value Work: 0.190
- R-Value Observed: 0.192
wwPDB Validation 3D Report Full Report
This is version 1.2 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Immunity 23 family protein | 172 | Burkholderia dolosa AU0158 | Mutation(s): 0 Gene Names: AK34_4389 |  | |
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| EPE Query on EPE | C [auth B] | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID C8 H18 N2 O4 S JKMHFZQWWAIEOD-UHFFFAOYSA-N |  | ||
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| MSE Query on MSE | A, B | L-PEPTIDE LINKING | C5 H11 N O2 Se |  | MET |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.50 Å
- R-Value Free: 0.205
- R-Value Work: 0.190
- R-Value Observed: 0.192
- Space Group: P 63
- Diffraction Data: https://doi.org/10.18430/M35FFP
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 116.298 | α = 90 |
| b = 116.298 | β = 90 |
| c = 70.065 | γ = 120 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| HKL-3000 | data reduction |
| HKL-3000 | data scaling |
| HKL-3000 | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2016-01-20 Deposition Author(s): Michalska, K., Stols, L., Eschenfeldt, W., Goulding, C.W., Joachimiak, A., Midwest Center for Structural Genomics (MCSG), Structure-Function Analysis of Polymorphic CDI Toxin-Immunity Protein Complexes (UC4CDI)
| Funding Organization | Location | Grant Number |
|---|---|---|
| National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) | United States | GM094585 |
| National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) | United States | GM102318 |
Revision History (Full details and data files)
- Version 1.0: 2016-01-20
Type: Initial release - Version 1.1: 2017-09-20
Changes: Author supporting evidence, Derived calculations - Version 1.2: 2019-12-25
Changes: Author supporting evidence
