Download MendeleyReversible S-Nitrosylation in an Engineered Mutant of Pseudomonas aeruginosa Azurin with Red Copper Site
Tian, S., Lu, Y.To be published.
4WKX
Reversible S-Nitrosylation in an Engineered Mutant of Pseudomonas aeruginosa Azurin with Red Copper Site
- PDB DOI: https://doi.org/10.2210/pdb4WKX/pdb
- Classification: OXIDOREDUCTASE
- Organism(s): Pseudomonas aeruginosa PAO1
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2014-10-03 Released: 2015-10-07
- Funding Organization(s): National Science Foundation (NSF, United States)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.94 Å
- R-Value Free: 0.235
- R-Value Work: 0.198
- R-Value Observed: 0.201
wwPDB Validation 3D Report Full Report
This is version 1.3 of the entry. See complete history.
Literature
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Azurin | 128 | Pseudomonas aeruginosa PAO1 | Mutation(s): 2 Gene Names: azu, PA4922 |  | |
UniProt | |||||
Find proteins for P00282 (Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)) Explore P00282 Go to UniProtKB: P00282 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P00282 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| TRS Query on TRS | D [auth A], G [auth B] | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL C4 H12 N O3 LENZDBCJOHFCAS-UHFFFAOYSA-O |  | ||
| CU Query on CU | C [auth A], E [auth A], F [auth B], H [auth B] | COPPER (II) ION Cu JPVYNHNXODAKFH-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.94 Å
- R-Value Free: 0.235
- R-Value Work: 0.198
- R-Value Observed: 0.201
- Space Group: P 31 2 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 47.774 | α = 90 |
| b = 47.774 | β = 90 |
| c = 241.641 | γ = 120 |
| Software Name | Purpose |
|---|---|
| HKL-2000 | data reduction |
| PHENIX | refinement |
| PDB_EXTRACT | data extraction |
| PHASER | phasing |
| DENZO | data reduction |
| SCALEPACK | data scaling |
Entry History & Funding Information
Deposition Data
| Funding Organization | Location | Grant Number |
|---|---|---|
| National Science Foundation (NSF, United States) | United States | CHE-1413328 |
Revision History (Full details and data files)
- Version 1.0: 2015-10-07
Type: Initial release - Version 1.1: 2017-09-13
Changes: Author supporting evidence, Derived calculations, Refinement description - Version 1.2: 2019-11-27
Changes: Author supporting evidence - Version 1.3: 2023-12-27
Changes: Data collection, Database references, Derived calculations
