Download MendeleyStructure-Based Enzyme Tailoring of 5-Hydroxymethylfurfural Oxidase
Dijkman, W.P., Binda, C., Fraaije, M.W., Mattevi, A.(2015) ACS Catal 5: 1833
4UDP
Crystal structure of 5-hydroxymethylfurfural oxidase (HMFO) in the oxidized state
- PDB DOI: https://doi.org/10.2210/pdb4UDP/pdb
- Classification: OXIDOREDUCTASE
- Organism(s): Methylovorus sp. MP688
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2014-12-11 Released: 2015-03-18
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.90 Å
- R-Value Free: 0.236
- R-Value Work: 0.178
- R-Value Observed: 0.181
This is version 1.2 of the entry. See complete history.
Literature
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| GLUCOSE-METHANOL-CHOLINE OXIDOREDUCTASE | 531 | Methylovorus sp. MP688 | Mutation(s): 0 |  | |
UniProt | |||||
Find proteins for E4QP00 (Methylovorus sp. (strain MP688)) Explore E4QP00 Go to UniProtKB: E4QP00 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | E4QP00 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| FAD Query on FAD | C [auth A], D [auth B] | FLAVIN-ADENINE DINUCLEOTIDE C27 H33 N9 O15 P2 VWWQXMAJTJZDQX-UYBVJOGSSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.90 Å
- R-Value Free: 0.236
- R-Value Work: 0.178
- R-Value Observed: 0.181
- Space Group: P 1 21 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 49.964 | α = 90 |
| b = 120.907 | β = 91.39 |
| c = 72.322 | γ = 90 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| XDS | data reduction |
| Aimless | data scaling |
| BALBES | phasing |
Entry History
Deposition Data
- Released Date: 2015-03-18 Deposition Author(s): Dijkman, W., Binda, C., Fraaije, M., Mattevi, A.
Revision History (Full details and data files)
- Version 1.0: 2015-03-18
Type: Initial release - Version 1.1: 2018-03-07
Changes: Data collection, Source and taxonomy - Version 1.2: 2023-12-20
Changes: Data collection, Database references, Derived calculations, Other, Refinement description
