Download MendeleyMultiple mechanisms render Erk proteins MEK-independent
Livnah, O., Karamansha, Y., Tzarum, N.To be published.
4S33
ERK2 R65S mutant complexed with AMP-PNP
- PDB DOI: https://doi.org/10.2210/pdb4S33/pdb
- Classification: TRANSFERASE
- Organism(s): Rattus norvegicus
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2015-01-26 Released: 2016-01-27
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.48 Å
- R-Value Free: 0.239
- R-Value Work: 0.196
- R-Value Observed: 0.199
This is version 1.1 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Mitogen-activated protein kinase 1 | 358 | Rattus norvegicus | Mutation(s): 1 Gene Names: Mapk1, Erk2, Mapk, Prkm1 EC: 2.7.11.24 |  | |
UniProt | |||||
Find proteins for P63086 (Rattus norvegicus) Explore P63086 Go to UniProtKB: P63086 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P63086 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| ANP Query on ANP | B [auth A] | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER C10 H17 N6 O12 P3 PVKSNHVPLWYQGJ-KQYNXXCUSA-N |  | ||
| SO4 Query on SO4 | C [auth A] | SULFATE ION O4 S QAOWNCQODCNURD-UHFFFAOYSA-L |  | ||
| MG Query on MG | D [auth A] | MAGNESIUM ION Mg JLVVSXFLKOJNIY-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.48 Å
- R-Value Free: 0.239
- R-Value Work: 0.196
- R-Value Observed: 0.199
- Space Group: P 1 21 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 48.813 | α = 90 |
| b = 70.438 | β = 109.28 |
| c = 60.082 | γ = 90 |
| Software Name | Purpose |
|---|---|
| HKL-2000 | data collection |
| AMoRE | phasing |
| REFMAC | refinement |
| HKL-2000 | data reduction |
| HKL-2000 | data scaling |
Entry History
Deposition Data
- Released Date: 2016-01-27 Deposition Author(s): Livnah, O., Karamansha, Y., Tzarum, N.
Revision History (Full details and data files)
- Version 1.0: 2016-01-27
Type: Initial release - Version 1.1: 2023-09-20
Changes: Data collection, Database references, Derived calculations, Refinement description
