Download MendeleyExpanding the drug metabolism function of P450BM3
Ren, X.K., Yorke, J.A., Taylor, A., Zhang, A.L., Zhang, T., Zhou, W.H., Wong, L.-L.To be published.
4RSN
Crystal structure of the E267V mutant of cytochrome P450 BM3
- PDB DOI: https://doi.org/10.2210/pdb4RSN/pdb
- Classification: OXIDOREDUCTASE
- Organism(s): Priestia megaterium
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2014-11-09 Released: 2015-12-02
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.70 Å
- R-Value Free: 0.207
- R-Value Work: 0.175
- R-Value Observed: 0.177
This is version 1.1 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Bifunctional P-450/NADPH-P450 reductase | 460 | Priestia megaterium | Mutation(s): 5 Gene Names: cyp102, cyp102A1, p450bm-3 EC: 1.14.14.1 |  | |
UniProt | |||||
Find proteins for P14779 (Priestia megaterium (strain ATCC 14581 / DSM 32 / CCUG 1817 / JCM 2506 / NBRC 15308 / NCIMB 9376 / NCTC 10342 / NRRL B-14308 / VKM B-512 / Ford 19)) Explore P14779 Go to UniProtKB: P14779 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P14779 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| HEM Query on HEM | C [auth A], D [auth B] | PROTOPORPHYRIN IX CONTAINING FE C34 H32 Fe N4 O4 KABFMIBPWCXCRK-RGGAHWMASA-L |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.70 Å
- R-Value Free: 0.207
- R-Value Work: 0.175
- R-Value Observed: 0.177
- Space Group: P 31
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 78.299 | α = 90 |
| b = 78.299 | β = 90 |
| c = 203.19 | γ = 120 |
| Software Name | Purpose |
|---|---|
| HKL-2000 | data collection |
| PHASES | phasing |
| PHENIX | refinement |
| HKL-2000 | data reduction |
| HKL-2000 | data scaling |
Entry History
Deposition Data
- Released Date: 2015-12-02 Deposition Author(s): Zhou, W.H., Zhang, A.L., Zhang, T., Ren, X.K., Yorke, J.A., Taylor, A., Wong, L.-L.
Revision History (Full details and data files)
- Version 1.0: 2015-12-02
Type: Initial release - Version 1.1: 2023-11-08
Changes: Data collection, Database references, Derived calculations, Refinement description
