4NQZ

Crystal Structure of the Pseudomonas aeruginosa Enoyl-Acyl Carrier Protein Reductase (FabI) in apo form

PDB DOI: https://doi.org/10.2210/pdb4NQZ/pdb

Classification: OXIDOREDUCTASE
Organism(s): Pseudomonas aeruginosa PAO1
Expression System: Escherichia coli
Mutation(s): No

Deposited: 2013-11-26 Released: 2014-11-26
Deposition Author(s): Chi, Y.M., Lee, J.H., Park, A.K.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.60 Å
R-Value Free: 0.286
R-Value Work: 0.199
R-Value Observed: 0.203

wwPDB Validation 3D Report Full Report

This is version 1.2 of the entry. See complete history.

Literature

Crystal Structures of Pseudomonas aeruginosa Enoyl-ACP Reductase (FabI) in the Presence and Absence of NAD+ and Triclosan

Lee, J.H., Park, A.K., Chi, Y.M., Jeong, S.W.

(2015) Bull Korean Chem Soc 36: 322-326

DOI: https://doi.org/10.1002/bkcs.10084

Macromolecule Content

Total Structure Weight: 465.71 kDa
Atom Count: 29,449
Modelled Residue Count: 3,926
Deposited Residue Count: 4,368
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI	A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P	273	Pseudomonas aeruginosa PAO1	Mutation(s): 0 Gene Names: fabI, PA1806 EC: 1.3.1.9
UniProt
Find proteins for Q9ZFE4 (Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)) Explore Q9ZFE4 Go to UniProtKB: Q9ZFE4
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q9ZFE4
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.60 Å
R-Value Free: 0.286
R-Value Work: 0.199
R-Value Observed: 0.203
Space Group: P 1 2₁ 1

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 117.322	α = 90
b = 155.844	β = 111.06
c = 129.448	γ = 90

Software Package:

Software Name	Purpose
PHENIX	refinement
PDB_EXTRACT	data extraction
HKL-2000	data collection
HKL-2000	data reduction
HKL-2000	data scaling
PHENIX	phasing

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2014-11-26

Deposition Author(s):

Revision History (Full details and data files)

Version 1.0: 2014-11-26
Type: Initial release
Version 1.1: 2015-05-06
Changes: Database references
Version 1.2: 2023-11-08
Changes: Data collection, Database references, Refinement description

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.