4MI2

Crystal structure of putative Enoyl-CoA hydratase/isomerase from Mycobacterium abscessus

PDB DOI: https://doi.org/10.2210/pdb4MI2/pdb

Classification: ISOMERASE
Organism(s): Mycobacteroides abscessus ATCC 19977
Expression System: Escherichia coli BL21(DE3)
Mutation(s): No

Deposited: 2013-08-30 Released: 2013-09-18
Deposition Author(s): Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.30 Å
R-Value Free: 0.213
R-Value Work: 0.179
R-Value Observed: 0.181

wwPDB Validation 3D Report Full Report

This is version 1.1 of the entry. See complete history.

Literature

Crystal structure of putative Enoyl-CoA hydratase/isomerase from Mycobacterium abscessus

Davies, D.R., Lukacs, C., Lorimer, D., Edwards, T.E.

To be published.

Macromolecule Content

Total Structure Weight: 82.1 kDa
Atom Count: 5,835
Modelled Residue Count: 782
Deposited Residue Count: 801
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Putative enoyl-CoA hydratase/isomerase	A, B, C	267	Mycobacteroides abscessus ATCC 19977	Mutation(s): 0 Gene Names: MAB_0905 EC: 5.3.3.18
UniProt
Find proteins for B1MJ43 (Mycobacteroides abscessus (strain ATCC 19977 / DSM 44196 / CCUG 20993 / CIP 104536 / JCM 13569 / NCTC 13031 / TMC 1543 / L948)) Explore B1MJ43 Go to UniProtKB: B1MJ43
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	B1MJ43
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.30 Å
R-Value Free: 0.213
R-Value Work: 0.179
R-Value Observed: 0.181
Space Group: P 4₁ 2₁ 2

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 94.92	α = 90
b = 94.92	β = 90
c = 195.37	γ = 90

Software Package:

Software Name	Purpose
PHASER	phasing
REFMAC	refinement

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2013-09-18

Deposition Author(s):

Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Revision History (Full details and data files)

Version 1.0: 2013-09-18
Type: Initial release
Version 1.1: 2024-02-28
Changes: Data collection, Database references

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.