4LKU

Structure of the C-terminal domain of the E. coli mechanosensitive channel of large conductance

PDB DOI: https://doi.org/10.2210/pdb4LKU/pdb

Classification: TRANSPORT PROTEIN
Organism(s): Escherichia coli DH1
Mutation(s): No

Deposited: 2013-07-08 Released: 2013-10-02
Deposition Author(s): Walton, T.A., Rees, D.C.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 1.45 Å
R-Value Free: 0.225
R-Value Work: 0.169
R-Value Observed: 0.172

wwPDB Validation 3D Report Full Report

This is version 1.3 of the entry. See complete history.

Literature

Structure and stability of the C-terminal helical bundle of the E. coli mechanosensitive channel of large conductance.

Walton, T.A., Rees, D.C.

(2013) Protein Sci 22: 1592-1601

PubMed: 24038743 Search on PubMedSearch on PubMed Central
DOI: https://doi.org/10.1002/pro.2360
Primary Citation of Related Structures:
4LKU

PubMed Abstract:
The crystal structure of the cytoplasmic domain (CTD) from the mechanosensitive channel of large conductance (MscL) in E. coli has been determined at 1.45 Å resolution. This domain forms a pentameric coiled coil similar to that observed in the structure of MscL from M. tuberculosis and also found in the cartilage oligomeric matrix protein (COMPcc). It contains canonical hydrophobic and atypical ionic interactions compared to previously characterized coiled coil structures. Thermodynamic analysis indicates that while the free EcMscL-CTD is less stable than other coiled coils, it is likely to remain folded in context of the full-length channel.

Organizational Affiliation

Division of Chemistry and Chemical Engineering, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California, 91125.

Macromolecule Content

Total Structure Weight: 16.19 kDa
Atom Count: 1,174
Modelled Residue Count: 130
Deposited Residue Count: 145
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Large-conductance mechanosensitive channel	A, B, C, D, E	29	Escherichia coli DH1	Mutation(s): 0
UniProt
Find proteins for P0A742 (Escherichia coli (strain K12)) Explore P0A742 Go to UniProtKB: P0A742
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P0A742
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 1.45 Å
R-Value Free: 0.225
R-Value Work: 0.169
R-Value Observed: 0.172
Space Group: P 1 2₁ 1

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 32.25	α = 90
b = 55.12	β = 93.58
c = 32.85	γ = 90

Software Package:

Software Name	Purpose
SCALA	data scaling
PHASER	phasing
PHENIX	refinement
PDB_EXTRACT	data extraction
Blu-Ice	data collection
MOSFLM	data reduction

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2013-10-02

Deposition Author(s):

Revision History (Full details and data files)

Version 1.0: 2013-10-02
Type: Initial release
Version 1.1: 2013-11-20
Changes: Database references
Version 1.2: 2017-11-15
Changes: Refinement description
Version 1.3: 2024-02-28
Changes: Data collection, Database references

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.