4KTS

Bovine trypsin in complex with microviridin J at pH 8.5


Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.30 Å
  • R-Value Free: 0.128 
  • R-Value Work: 0.106 
  • R-Value Observed: 0.107 

wwPDB Validation   3D Report Full Report


This is version 1.2 of the entry. See complete history


Literature

Harnessing the evolvability of tricyclic microviridins to dissect protease-inhibitor interactions.

Weiz, A.R.Ishida, K.Quitterer, F.Meyer, S.Kehr, J.C.Muller, K.M.Groll, M.Hertweck, C.Dittmann, E.

(2014) Angew Chem Int Ed Engl 53: 3735-3738

  • DOI: https://doi.org/10.1002/anie.201309721
  • Primary Citation of Related Structures:  
    4KTS, 4KTU

  • PubMed Abstract: 

    Understanding and controlling proteolysis is an important goal in therapeutic chemistry. Among the natural products specifically inhibiting proteases microviridins are particularly noteworthy. Microviridins are ribosomally produced and posttranslationally modified peptides that are processed into a unique, cagelike architecture. Here, we report a combined rational and random mutagenesis approach that provides fundamental insights into selectivity-conferring moieties of microviridins. The potent variant microviridin J was co-crystallized with trypsin, and for the first time the three-dimensional structure of microviridins was determined and the mode of inhibition revealed.


  • Organizational Affiliation

    Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Strasse 24-25, 14476 Potsdam-Golm (Germany).


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Cationic trypsin223Bos taurusMutation(s): 0 
EC: 3.4.21.4
UniProt
Find proteins for P00760 (Bos taurus)
Explore P00760 
Go to UniProtKB:  P00760
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP00760
Sequence Annotations
Expand
  • Reference Sequence

Find similar proteins by:  Sequence   |   3D Structure  

Entity ID: 2
MoleculeChains Sequence LengthOrganismDetailsImage
Microviridin14Microcystis aeruginosa MRCMutation(s): 1 
Gene Names: mdnA
UniProt
Find proteins for B2G3C8 (Microcystis aeruginosa MRC)
Explore B2G3C8 
Go to UniProtKB:  B2G3C8
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupB2G3C8
Sequence Annotations
Expand
  • Reference Sequence
Small Molecules
Biologically Interesting Molecules (External Reference) 1 Unique
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.30 Å
  • R-Value Free: 0.128 
  • R-Value Work: 0.106 
  • R-Value Observed: 0.107 
  • Space Group: P 21 21 21
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 57.33α = 90
b = 64.49β = 90
c = 71.21γ = 90
Software Package:
Software NamePurpose
XDSdata scaling
PHASERphasing
REFMACrefinement
XDSdata reduction
XSCALEdata scaling

Structure Validation

View Full Validation Report



Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2014-04-02
    Type: Initial release
  • Version 1.1: 2014-04-16
    Changes: Database references
  • Version 1.2: 2023-09-20
    Changes: Data collection, Database references, Derived calculations, Refinement description