4JJR

A P21 crystal form of mammalian casein kinase 1 delta


Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 2.41 Å
  • R-Value Free: 0.237 
  • R-Value Work: 0.210 
  • R-Value Observed: 0.211 

wwPDB Validation   3D Report Full Report


This is version 1.5 of the entry. See complete history


Literature

A monoclinic crystal form of casein kinase 1 delta

Zeringo, N.A.Murphy, L.McCloskey, E.A.Rohal, L.Bellizzi, J.J.

(2013) Acta Crystallogr Sect F Struct Biol Cryst Commun 69: 1077-1083

  • DOI: https://doi.org/10.1107/S1744309113023403
  • Primary Citation of Related Structures:  
    4JJR

  • PubMed Abstract: 

    Casein kinase 1 δ (CK1δ) is a regulatory enzyme in the mammalian circadian oscillator and represents a potential pharmacological target for modulating circadian rhythms. Crystal structures of four different polymorphs of CK1δ have previously been determined and this article reports the crystallization and structure determination of a new crystal form belonging to space group P21. Comparison of CK1δ crystal structures reveals few conformational differences within the C-terminal lobe, but more significant movements of the β-sheet region of the N-terminal lobe were observed.


  • Organizational Affiliation

    Department of Chemistry, University of Toledo, 2801 West Bancroft Street, Toledo, OH 43606, USA.


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Casein kinase I isoform delta
A, B
299Mus musculusMutation(s): 0 
Gene Names: Csnk1dHckid
EC: 2.7.11.1 (PDB Primary Data), 2.7.11.26 (PDB Primary Data)
UniProt
Find proteins for Q9DC28 (Mus musculus)
Explore Q9DC28 
Go to UniProtKB:  Q9DC28
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupQ9DC28
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 2.41 Å
  • R-Value Free: 0.237 
  • R-Value Work: 0.210 
  • R-Value Observed: 0.211 
  • Space Group: P 1 21 1
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 52.145α = 90
b = 102.251β = 101.53
c = 64.593γ = 90
Software Package:
Software NamePurpose
SCALEPACKdata scaling
PHASERphasing
PHENIXrefinement
PDB_EXTRACTdata extraction
HKL-2000data collection
HKL-2000data reduction

Structure Validation

View Full Validation Report



Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2013-06-12
    Type: Initial release
  • Version 1.1: 2013-06-19
    Changes: Structure summary
  • Version 1.2: 2013-08-21
    Changes: Data collection, Derived calculations
  • Version 1.3: 2013-10-09
    Changes: Database references
  • Version 1.4: 2013-10-16
    Changes: Database references
  • Version 1.5: 2023-09-20
    Changes: Data collection, Database references, Refinement description