Download MendeleyStructure of Clostridium difficile PilJ Exhibits Unprecedented Divergence from Known Type IV Pilins.
Piepenbrink, K.H., Maldarelli, G.A., de la Pena, C.F., Mulvey, G.L., Snyder, G.A., De Masi, L., von Rosenvinge, E.C., Gunther, S., Armstrong, G.D., Donnenberg, M.S., Sundberg, E.J.(2014) J Biol Chem 289: 4334-4345
- PubMed: 24362261
- DOI: https://doi.org/10.1074/jbc.M113.534404
- Primary Citation of Related Structures:
4IXJ - PubMed Abstract:
Type IV pili are produced by many pathogenic Gram-negative bacteria and are important for processes as diverse as twitching motility, cellular adhesion, and colonization. Recently, there has been an increased appreciation of the ability of Gram-positive species, including Clostridium difficile, to produce Type IV pili. Here we report the first three-dimensional structure of a Gram-positive Type IV pilin, PilJ, demonstrate its incorporation into Type IV pili, and offer insights into how the Type IV pili of C. difficile may assemble and function. PilJ has several unique structural features, including a dual-pilin fold and the incorporation of a structural zinc ion. We show that PilJ is incorporated into Type IV pili in C. difficile and present a model in which the incorporation of PilJ into pili exposes the C-terminal domain of PilJ to create a novel interaction surface.
Organizational Affiliation:
From the Institute of Human Virology.
