Download MendeleyG215S, A251G, T257A, D260G, T262D mutant of carboxypeptidase T from Thermoactinomyces vulgaris with N-BOC-L-Leu
Timofeev, V.I., Kuznetsov, S.A., Akparov, V.K., Kuranova, I.P.To be published.
4IK2
G215S, A251G, T257A, D260G, T262D mutant of carboxypeptidase T from Thermoactinomyces vulgaris with N-BOC-L-Leu
- PDB DOI: https://doi.org/10.2210/pdb4IK2/pdb
- Classification: HYDROLASE
- Organism(s): Thermoactinomyces vulgaris
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2012-12-25 Released: 2013-12-25
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.40 Å
- R-Value Free: 0.136
- R-Value Work: 0.125
- R-Value Observed: 0.125
This is version 1.1 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Carboxypeptidase T | 326 | Thermoactinomyces vulgaris | Mutation(s): 5 Gene Names: cpt EC: 3.4.17.18 |  | |
UniProt | |||||
Find proteins for P29068 (Thermoactinomyces vulgaris) Explore P29068 Go to UniProtKB: P29068 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P29068 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 4 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| BL2 Query on BL2 | D [auth A] | N-(tert-butoxycarbonyl)-L-leucine C11 H21 N O4 MDXGYYOJGPFFJL-QMMMGPOBSA-N |  | ||
| SO4 Query on SO4 | B [auth A] | SULFATE ION O4 S QAOWNCQODCNURD-UHFFFAOYSA-L |  | ||
| ZN Query on ZN | C [auth A] | ZINC ION Zn PTFCDOFLOPIGGS-UHFFFAOYSA-N |  | ||
| CA Query on CA | E [auth A], F [auth A], G [auth A], H [auth A] | CALCIUM ION Ca BHPQYMZQTOCNFJ-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.40 Å
- R-Value Free: 0.136
- R-Value Work: 0.125
- R-Value Observed: 0.125
- Space Group: P 63 2 2
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 158.243 | α = 90 |
| b = 158.243 | β = 90 |
| c = 104.657 | γ = 120 |
| Software Name | Purpose |
|---|---|
| HKL-2000 | data collection |
| PHASER | phasing |
| REFMAC | refinement |
| HKL-2000 | data reduction |
| HKL-2000 | data scaling |
Entry History
Deposition Data
- Released Date: 2013-12-25 Deposition Author(s): Timofeev, V.I., Kuznetsov, S.A., Akparov, V.K., Kuranova, I.P.
Revision History (Full details and data files)
- Version 1.0: 2013-12-25
Type: Initial release - Version 1.1: 2023-11-08
Changes: Data collection, Database references, Derived calculations, Experimental preparation, Refinement description
